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在实施轮状病毒疫苗之前和之后,在委内瑞拉引起婴儿急性胃肠炎的人类腺病毒的分子表征

Ruth Blanco¹, Ana C Alcalá², Rixio Fernández¹

¹Laboratorio de Biología de Virus, Centro de Microbiología y Biología Celular (CMBC), Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.

Diagnostic microbiology and infectious disease

|September 8, 2023

概括

人类腺病毒 (HAdV) 在委内瑞拉引起了显著的儿科胃肠炎. 在接种疫苗的儿童中增加了HAdV-F检测,这凸显了除了轮状病毒之外需要更广泛的病毒监测.

科学领域:

病毒学病毒学
公共卫生公共卫生
儿科儿科儿科

背景情况:

人类腺病毒 (HAdV) 种F是儿科急性胃肠炎 (AGE) 的常见原因.
委内瑞拉HAdV的流行病学影响在很大程度上仍未被描述.
了解HAdV患病率对于公共卫生战略至关重要.

研究的目的:

确定HAdV在委内瑞拉患有AGE的儿童中的流行率和分子多样性.
分析随着时间的推移和与疫苗接种状态相关的HAdV检测趋势.
提供有关流传的HAdV菌株的遗传关系的见解.

主要方法:

在3个委内瑞拉城市 (2001-2013) 的AGE儿童的630个便样本中检测出HAdV的PCR检测.
限制酶分析 (REA) 用于物种和类型识别 (F40/41).
对28个HAdV基因组的遗传学分析.

主要成果:

在19.5%的病例中检测到HAdV,主要是24个月以下的女性门诊患者.
在研究期间,观察到HAdV检测率的显著上升趋势.
在接种轮状病毒疫苗 (28.4%) 的儿童中,检测率明显高于未接种疫苗 (9.5%) 的儿童.
HAdV-F,主要是41型 (79.8%),显示全球遗传相似性,并形成了两个主要的分类.

结论:

HAdV,特别是F41型,在委内瑞拉的儿科胃肠炎中起着重要作用.
这些发现强调了与轮状病毒一起监测HAdV的重要性,特别是在疫苗引入后.
建议对各种病毒病原体进行积极监测,以有效管理公共卫生.

关键词:

遗传的变异性遗传的变异性人类腺核病毒人类腺核病毒.分子类型的定型.儿童急性胃肠炎的情况

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