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从尿路中分离出来的Cyberlindnera fabianii菌株的鉴定,分子表征和抗真菌敏感性

Fatma Mutlu Sariguzel¹, Gamze Kalin Unuvar², Osman Kucukoglu¹

¹Department of Medical Microbiology, Faculty of Medicine, Erciyes University, Kayseri 38030, Turkey.

Journal de mycologie medicale

|September 8, 2023

概括

准确识别Cyberlindnera fabianii,一个尿路病原体经常被误认为是Candida utilis,至关重要. 像序列分析和MALDI-TOF MS等分子方法正确识别了Cy. 弗拉比安尼,显示出对可纳的耐药性.

科学领域:

医学真菌学医学真菌学
临床微生物学临床微生物学
传染性疾病传染性疾病

背景情况:

网络虫 (Cyberlindnera fabianii) 是一种机会性病原体.
现型识别方法可能会错误地识别 Cy. 作为使用的Candida. fabianii.
准确的鉴定对于有效治疗尿路感染至关重要.

研究的目的:

为了准确识别Cy. 从尿路感染引起的Fabianii菌株.
描述这些菌株的分子特征.
为了确定它们对各种抗真菌剂的敏感性.

主要方法:

使用了表型鉴定,序列分析 (ITS1-2,D1/D2,EF-1-alpha) 和MALDI-TOF MS.
用邻居连接方法进行了植物遗传学分析.
对9种抗真菌药物的抗真菌敏感性测试遵循CLSI M60-Ed2的建议.

主要成果:

所有29个菌株在表型上都被确定为C. utilis,但分子方法证实它们是Cy. 法比安尼. 法比安尼. 法比安尼.
对核糖体DNA基因区域的序列分析显示,没有物种内核酸变异.
与其他测试的抗真菌药物相比,可纳的MIC50/MIC90值较高.

结论:

这是Cy.Cy. 应考虑在抗可纳的尿路酵母菌感染中使用 fabianii.
传统的表型方法不足以准确的Cy. 法比安尼的身份证.
序列分析和MALDI-TOF MS提供了可靠的Cy.的识别. 法比安尼. 法比安尼. 法比安尼.

关键词:

抗真菌易感性抗真菌易感性在Candida的使用中.网络监视者法比安尼马尔迪-托夫 (MALDI-TOF) 是一个国家元首.人类遗传学分析序列分析是指进行序列分析.

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