Usp25-Erlin1/2的活性限制胆固醇流量,以限制病毒感染
Qi Wen Teo1, Ho Him Wong2, Tiaan Heunis3
1HKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Developmental cell
|September 8, 2023
概括
杜比基酶 Usp25 通过控制脂质代谢来限制病毒感染. 丢失USP25会增加病毒的产生,改变胆固醇的流量,影响免疫反应.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 对感染的免疫反应涉及显著的代谢重编程.
- 感染期间脂质代谢途径调节的精确机制尚未完全理解.
- 细胞双化酶 (DUBs) 参与免疫调节,但它们在病毒感染中的具体作用往往不清楚.
研究的目的:
- 研究duebiquitylase Usp25在宿主对流感病毒感染的反应中的作用.
- 阐明 Usp25 调节脂质代谢和抗病毒免疫力的分子机制.
主要方法:
- 使用了USP25删除的人类肺上皮层A549细胞.
- 进行了野生类型和催化无活性 Usp25.25 的病毒生产测定和救援实验.
- 进行蛋白质组学分析以确定 Usp25 相互作用体和基板.
- 评估了Srebp2激活,胆固醇流动和托尔样受体3 (TLR3) 相关的反应.
主要成果:
- 删除了USP25的细胞显示流感病毒的产生增加了10倍以上,这被野生型USP25所拯救.
- 埃尔林1/2被确定为 Usp25 的基质,其降解发生在 Usp25 缺乏细胞中.
- Usp25的丧失导致了Srebp2的激活,增加了胆固醇的流动,并削弱了TLR3介导的抗病毒反应.
结论:
- Usp25作为一种限制病毒复制的关键宿主因子起作用.
- Usp25通过Erlin1/2降解来调节脂质生物合成和胆固醇流量.
- 这种Usp25对脂质代谢的重编程对于建立有效的炎症和抗病毒反应至关重要.
相关概念视频
Cholesterol: Significance and Regulation
580
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Considering cholesterol and...
580
Retroviruses
12.4K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.4K
Assembly of the Lipid Bilayer in the ER
3.2K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.2K


