具有多功能聚亲和力和结合强度的自然灵感材料结合
Francisco G Blanco1, Rainhard Machatschek2, Manuela Keller2
1Polymer Biotechnology Group, Plant and Microbial Biotechnology Department, Margarita Salas Centre for Biological Research (CIB - CSIC), Madrid, Spain; Interdisciplinary Platform of Sustainable Plastics towards a Circular Economy, Spanish National Research Council (SusPlast-CSIC), Madrid, Spain.
International journal of biological macromolecules
|September 8, 2023
概括
研究人员设计的小型材料结合 (MBPs) 灵感来自phasins,以功能化生物降解的聚合物,如聚酸酸盐 (PHAs). 这些体显示出高亲和力结合,使货物蛋白固定和合成聚修饰的潜在应用.
科学领域:
- 生物材料科学是生物材料的科学.
- 聚合物化学 聚合物化学
- 蛋白质工程是一种蛋白质工程.
背景情况:
- 可生物降解的聚合物,包括聚酸酸盐 (PHAs),在生物医学中至关重要,但缺乏针对特定应用的固有功能组.
- 本地GAPs (例如,phasins) 具有聚合物结合和表面活性物质的特性,为表面功能化提供了一个模型.
研究的目的:
- 设计用于功能化聚表面的小型材料结合 (MBPs).
- 评估这些MBP与不同PHA的结合亲和力和特异性.
- 证明MBP对固定载荷蛋白和修改聚表面的有用性.
主要方法:
- 基于Pseudomonas putida的相素 (PhaF和PhaI) 的两性性的合理设计,导致MinP和MinI.
- 在体内光研究以评估结选择性.
- 在体外结合试验中,使用兰迈尔-布洛杰特技术和圆测量来确定解离常数 (KD).
- 原子力显微镜 (AFM) 和布鲁斯特角显微镜 (BAM) 用于分析表面形态变化.
主要成果:
- 工程化 (MinP和MinI) 在体外显示高亲和度结合 (nM KD) 到PHB和PHOH聚合物.
- 观察到选择性活体内与PHOH的结合.
- 在吸附后,PHOH表面的显著形态变化.
- 使用MBP,成功将货物蛋白固定在聚表面上.
- 此外,MBPs还显示与聚乙烯四甲酸盐 (PET) 结合.
结论:
- 在试验室中,重新设计相位两性产生了高亲和度,尽管特异性较低的聚合物MBP.
- 这些MBP可以有效地功能化PHA表面,并固定货物蛋白.
- 证明与PET的结合表明了更广泛的修改合成聚合物的潜力.
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