特兰克萨姆酸会通过体细胞的亡引起的冠状体损伤,通过激活内分泌网膜应激诱导的冠状体损伤
Jiahao Wang1, Shuailong Liang1, Tianliang Ma1
1Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, China; Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, Xiangya Hospital, Central South University, Changsha, China.
概括
高度的三胺酸 (TXA) 对细胞具有细胞毒性,通过内细胞网膜应激诱导亡. 在关节手术时,建议度低于50 mg/mL,以防止软骨损伤.
科学领域:
- 生物医学科学 生物医学科学
- 整形外科 整形外科 整形外科
- 细胞生物学 细胞生物学
背景情况:
- 特兰胺酸 (TXA) 广泛用于关节手术,以减少出血.
- 在不同度下,局部TXA对软骨和软骨细胞的安全性尚不清楚.
- 了解TXA的细胞毒性潜力对于优化其临床应用至关重要.
研究的目的:
- 评估特兰胺酸 (TXA) 对软骨细胞和软骨的细胞毒性作用.
- 为了阐明TXA诱导的冠状细胞亡的潜在机制.
- 确定TXA在关节手术中的安全度极限.
主要方法:
- 在体外状细胞活力和亡测定.
- 在TXA治疗后的体内软骨形态和内容分析.
- 检测反应性氧物种,线粒体膜潜力和内质网膜压力 (ERS) 标志物.
- 流细胞计和西部斑点分析以检测亡和ERS相关蛋白质.
主要成果:
- TXA证明了剂量依赖的细胞毒性作用,在50 mg/mL和100 mg/mL时观察到显著的毒性.
- 通过反应性氧物种生成和线粒体功能障碍,TXA诱导了冠状细胞亡.
- TXA触发了过载和内质网膜应激 (ERS) 激活,这些都与亡有关.
- 亡和ERS抑制剂部分逆转了TXA诱导的冠状细胞死亡.
结论:
- 特兰胺酸 (TXA) 在50毫克/毫升及以上的度下对雄性细胞具有细胞毒性,主要是通过通过ERS激活诱导亡.
- 这些发现表明,在关节内应用中,TXA的使用应限制在50 mg/mL以下的度.
- 这项研究为完善关于在骨科手术中使用TXA来预防软骨损伤的临床指南提供了关键数据.
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