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在垃圾填埋场中的抗生素耐药性基因和病原体的微塑料丰富特性

Cheng Qiu¹, Yiwei Zhou¹, Hua Wang²

¹School of Civil Engineering and Architecture, Zhejiang University of Science and Technology, Hangzhou, 310023, Zhejiang, China.

|September 8, 2023

概括

垃圾填埋场中的微塑料含有抗生素耐药基因和病原体. 这些微塑料 (MP) 选择性地丰富特定的细菌,并对环境健康构成风险.

科学领域:

环境科学环境科学
微生物学微生物学
环境化学环境化学

背景情况:

微塑料 (MP) 污染对水生生态系统构成重大威胁.
垃圾填埋场液是MPs和抗生素耐药基因 (ARG) 的主要来源.
有限的研究存在于ARG和病原体殖民于浸出物中的MP.

研究的目的:

调查ARG和病原体在垃圾填埋液中化的聚乙烯二甲 (PET) 和聚烯 (PP) MPs上的殖民.
分析液年龄对MP相关细菌群落和ARG丰富的影响.
评估在水中的MP中发现的病原体所带来的健康风险.

主要方法:

在老化 (AL) 和预老化 (PL) 垃圾填埋场液中使用PET和PPMP的化实验.
使用表面特征化技术对MPs上的细菌细胞殖民和表面功能群的分析.
实时PCR测量MP的特定ARG (tetM,tetC,mcr-1,aac(6') -Ib-cr,blaTEM,blaSHV) 的数量.
16S rRNA基因测序以确定MP和液中的细菌社区多样性和组成.
病原体识别和量化MP和浸出物中的病原体.

主要成果:

在MPs上的细菌殖民增加了其表面的氧化功能组.
国会议员在液中选择性地丰富了特定的ARG.
在AL溶液中,MPs上的细菌多样性增加,但在PL溶液中降低.
MP类型影响了细菌群体的组成,在Chloroflexi,Acidobacteriota和Firmicutes中发生了显著的变化.
病原体,特别是与sul1和sul2基因相关的病原体,在MP上比周围的液中更丰富.

结论:

在垃圾填埋场的液中,MPs作为ARG和病原体的载体.
浸出物年龄和MP类型显著影响微生物殖民和ARG丰富.
这些发现突出了与垃圾填埋场中的国会议员相关的潜在环境健康风险.

关键词:

在 ARG 中,有 ARG 的存在.细菌社区的细菌社区垃圾填埋场的洗液是如何处理的微塑料是一种微塑料.病原体是一种病原体.

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