结构趋同赋予核运输受体Kap114p一个转录抑制器功能,对TATA结合蛋白进行结合
Chung-Chi Liao1,2, Yi-Sen Wang2, Wen-Chieh Pi3
1Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and National Defense Medical Center, Taipei, 11490, Taiwan.
Nature communications
|September 8, 2023
概括
核运输受体如卡里奥菲林-β (Kap-β) 对于基因调节至关重要. 这项研究揭示了Kap114p如何结合酵母TATA盒结合蛋白 (TBP) 来控制转录和应激反应.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 塔塔盒结合蛋白 (TBP) 调节核内的基因表达.
- 酵母中的核运输受体,称为酵母中的卡里奥菲林-β (Kap-β),促进TBP的核进口和功能.
- 以前的工作表明Kap114p,一个Kap-β,影响TBP依赖转录,但其精确的相互作用机制是未知的.
研究的目的:
- 阐明Kap114p与酵母TBP (yTBP) 相互作用的结构基础.
- 了解这种相互作用如何有助于调节yTBP的转录活动和细胞功能.
主要方法:
- 使用单粒子冷电子显微镜 (cryo-EM) 来确定Kap114p-yTBP复合物的结构.
- 进行了生物化学测试,以评估Kap114p-yTBP相互作用对转录和细胞反应的功能后果.
主要成果:
- 冷EM结构显示,Kap114p围绕着yTBP (yTBPC) 核心域的N端叶.
- 这种结合模式在结构上类似于与TBP相互作用的其他转录调节剂,这表明了融合进化.
- Kap114p被证明可以封存yTBP,防止其与促进体过早结合,并维持环境应激适应所必需的转录动态.
结论:
- 核运输受体,以Kap114p为例,是转录调节网络的组成部分.
- 对Kap114p-yTBP相互作用的结构和功能洞察力突出了调节基因表达和细胞适应性的新机制.
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