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一个染色体水平的基因组组合的黑色barbel鱼Pelteobagrus vachelli
Gaorui Gong1, Wensi Ke1, Qian Liao1
1Hubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
Scientific data
|September 8, 2023
概括
使用先进的测序,创建了一个高质量的黑鱼 (Pelteobagrus vachelli) 的基因组组. 这一资源将促进这一重要的水产养殖物种的遗传研究和育种计划.
科学领域:
- 水产养殖基因组学 水产养殖基因组学
- 进行比较基因组学.
- 动物遗传学动物遗传学
背景情况:
- 黑鱼 (Pelteobagrus vachelli) 由于其快速生长,对中国的水产养殖至关重要,特别是在混合黄色鱼生产中.
- 对P. vachelli的有限基因组信息阻碍了遗传研究和育种工作.
研究的目的:
- 为了生成一个高质量的染色体层次的基因组组合,黑色鱼 (Pelteobagrus vachelli).
- 为鱼的遗传改进和进化研究提供一个基础资源.
主要方法:
- 利用PacBio长读测序和Hi-C技术进行基因组组装.
- 采用生物信息学分析来评估基因组大小,基因含量,重复元素和组装完整性.
主要成果:
- 实现了692.10 Mb的染色体水平组合,其中99.9%的序列在26个染色体上.
- 确定了22,109个蛋白质编码基因,其中96.1%的功能注释和BUSCO得分为99.07%.
- 重复的元素约占基因组的35.79%.
结论:
- 高质量的P. vachelli基因组组合是鱼基因组学的一个重大进步.
- 这一资源将有助于未来在杂交鱼繁殖,比较基因组学和进化生物学方面的研究.
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