丸激素可以确定莫哈维沙漠海 (Gopherus agassizii) 的化性别
M A Walden1,2, Kevin J Loope3,4, Elizabeth A Hunter5
1Department of Natural Resources and Environmental Science, University of Nevada, Reno, Reno, NV, USA. mar.walden@gmail.com.
Scientific reports
|September 8, 2023
概括
血中的水平准确地确定了年轻的莫哈维沙漠海 (Gopherus agassizii) 的性别. 这种非侵入性方法有助于保护这一危物种的保护工作.
科学领域:
- 类学 类学 类学
- 保护生物学 保护生物学
- 内分泌学 在内分泌学.
背景情况:
- 蒙哈维沙漠海 (Gopherus agassizii) 是一个临灭绝的物种,表现出温度依赖的性别决定.
- 幼在外表上是性单态的,因此在现场接触时需要使用非手术性性别识别方法.
- 尽量减少处理对于野生动物保护至关重要.
研究的目的:
- 开发一种非手术方法,用于0-3个月大的莫哈韦沙漠海幼的性别鉴定.
- 评估血中丸激素水平是否可以在幼中区分雄性和雌性.
- 评估卵泡刺激激素 (FSH) 注射在帮助性别识别中的有效性.
主要方法:
- 在0-3个月大的被囚禁化幼中,使用酶链接免疫吸收试验 (ELISA) 量化了血激素.
- 性别通过大脑镜 (腹腔镜) 手术得到验证.
- 使用逻辑正常分布和参数引导来分析丸激素度,以估计误识率.
主要成果:
- 血激素量化准确地区分了幼的性别,误识率低 (原始样本的0.01-0.04%).
- 毛囊刺激激素 (FSH) 注射显示,与原始样本相比,错误识别率 (1.26-1.39%) 更高.
- 小血量 (0.1毫升) 足以准确量化.
结论:
- 血中丸激素度是一种可行的,高度准确的方法,用于在年轻的莫哈维沙漠海中识别性别.
- 这种非侵入性技术可以通过在野生种群中进行性别比率研究来支持保护.
- 该方法尽量减少处理压力,这对于保护危物种至关重要.
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