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Updated: Jul 1, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
00:09

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

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克隆动力学限制了在异种移植瘤中的选择检测CRISPR/Cas9屏幕

Tet Woo Lee1,2, Francis W Hunter3,4,5, Peter Tsai4,6

  • 1Auckland Cancer Society Research Centre, University of Auckland, Auckland, New Zealand. tw.lee@auckland.ac.nz.

Cancer gene therapy
|September 8, 2023
PubMed
概括

在体内优化CRISPR屏幕需要仔细考虑实验参数. 早期瘤采集和较小的引导RNA库大小提高了癌症模型中基因功能评估的可靠性.

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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...