在Pasteurella multocida感染期间,RACK1调解NLRP3炎症酶激活
Jinrong Ran1, Hang Yin1, Yating Xu1
1Joint International Research Laboratory of Animal Health and Animal Food Safety, College of Veterinary Medicine, Southwest University, Chongqing, 400715, China.
Veterinary research
|September 8, 2023
概括
激活的C激酶1 (RACK1) 的受体在帕斯图雷拉多种菌感染期间促进NLRP3炎症酶的激活. RACK1调节NF-κB并促进炎症酶组合,影响宿主对这种细菌的防御.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 细胞生物学 细胞生物学
背景情况:
- 帕斯托雷拉多菌 (Pm) 引起重要的动物疾病.
- NLRP3炎症酶对宿主防御微生物感染至关重要.
- 之前的研究表明PmCQ2激活NLRP3,但机制尚不清楚.
研究的目的:
- 阐明PmCQ2诱导的NLRP3炎症酶激活的机制.
- 在这个过程中研究活性C激酶1 (RACK1) 受体的作用.
主要方法:
- 使用初级小鼠腹膜巨细胞和小鼠组织.
- 采用诸如siRNA敲除,等离子体转染,免疫光染色和免疫沉等技术.
- 评估NLRP3炎症酶激活标志物,包括IL-1β,caspase-1,ASC斑点和NF-κB激活.
主要成果:
- 在PmCQ2感染下调RACK1表达.
- 过度表达RACK1可以防止PmCQ2诱导的细胞死亡,并减少细菌负载.
- RACK1的淘汰减弱了NLRP3炎症酶激活,而RACK1的过度表达促进了它.
- 发现RACK1与NLRP3和NEK7进行局部化和相互作用,这取决于流量.
结论:
- RACK1对于PmCQ2诱导的NLRP3炎症酶激活是必不可少的.
- 通过调节NF-κB信号传递,RACK1促进NLRP3炎症酶组合和激活.
- 在宿主对P. multocida感染的反应中,RACK1起着至关重要的作用.
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