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比较转录组学识别了参与绵羊 (Ovis aries) 生殖的关键垂体循环RNA

Jianqi Yang¹, Jishun Tang², Xiaoyun He¹

¹State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China.

Animals : an open access journal from MDPI

|September 9, 2023

概括

下垂体圆形RNAs (circRNAs) 在高生育率和低生育率的羊之间存在差异,影响生殖特征. 这些发现为羊的生育能力和脑垂体功能提供了新的见解.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
生殖生物学生殖生物学

背景情况:

循环RNAs (circRNAs) 是生物过程中的关键调节分子.
之前的研究在绵羊垂体中确定了circRNAs,但它们在变化生育能力中的作用仍未得到充分研究.
该FecB基因影响小尾汉羊的生育能力.

研究的目的:

为了研究脑下垂体中的circRNA表达特征FecB ++基因型小尾汉羊具有不同的生育能力.
为了识别与生殖阶段和生育相关的差异表达的circRNAs (DE circRNAs).
探索这些DE circRNAs的功能作用和调节网络.

主要方法:

在12个代表不同生育水平和雌性阶段的绵羊垂体样本上进行了RNA测序.
生物信息分析用于识别和量化circRNAs,并检测差异表达的circRNAs.
功能丰富分析和竞争的内源RNA (ceRNA) 网络构建被用来理解circRNA的功能.

主要成果:

总共有34,878个circRNAs被确定.
在多卵性 (高生育率) 和单卵性 (低生育率) 绵羊之间,在卵泡 (300 DE circRNAs) 和形 (347 DE circRNAs) 阶段,观察到circRNA表达的显著差异.
关键的DE circRNA与皮质醇合成和雌激素信号传导等途径有关,在ceRNA网络中确定了特定的circRNA,其目标是像oar-miR-432和oar-miR-370-3p这样的miRNA.

结论:

下垂体circRNA表达模式与绵羊的生育能力和雌性周期相相关.
DE circRNAs可能在调节垂体功能和生殖过程中发挥作用,可能是通过与类固醇激素的相互作用.
这项研究提供了关于circRNA功能及其对FecB++基因型绵羊生育能力的贡献的新鲜信息.

关键词:

圆形的RNAs是圆形的RNAs.pituitary pituitary pituitary pituitary pituitary pituitary pituitary pituitary pituitary pituitary 下垂体下垂体下垂体下垂体下垂体下垂体下垂体下垂体生殖生殖的繁殖.羊群羊群羊群的羊群是什么意思

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