转录组测序揭示了内源逆转录病毒chERV3的完整表达在体外可能会阻断关键的先天免疫通路
Xi Zhang1,2, Tingting Xie1,2, Xiaoqi Li1,2
1Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Animals : an open access journal from MDPI
|September 9, 2023
概括
像chERV3这样的内源逆转录病毒 (ERV) 在宿主基因组中活跃. 这项研究表明chERV3可能会抑制Toll-like受体通路,可能有助于病毒感染期间的免疫逃避.
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
背景情况:
- 内源逆转录病毒 (ERV) 是脊椎动物基因组中集成的病毒序列.
- 在初步分析中,在染色体1上发现了活性chERV3.
- 对于chERV3在宿主抗病毒反应中的作用尚不清楚.
研究的目的:
- 为了研究chERV3在宿主病毒感染的先天免疫反应中的功能.
- 为了确定chERV3是否影响免疫信号通路.
主要方法:
- 在体外制造了一个全长的chERV3逆克隆等离子体.
- 使用ELISA测量p27病毒颗粒含量.
- 进行了转录组分析 (RNA-Seq),以识别差异表达基因 (DEG) 和丰富的通路.
主要成果:
- 发现chERV3有可能产生p27病毒颗粒.
- 在传染后24小时和48小时观察到大量上下调节的DEG.
- 基因本体学和KEGG通路分析显示,在下调 DEGs 中,免疫相关过程的丰富,包括托尔类受体信号通路.
- 基因组丰富分析 (GSEA) 表示通过chERV3.3抑制托尔类受体信号通路.
结论:
- chERV3可能会产生病毒颗粒并影响宿主天生的免疫力.
- 似乎chERV3抑制了托尔类受体信号通路.
- 这种抑制表明chERV3在病毒感染期间的免疫逃避机制.
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