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从普通和软面包皮中获得的黑色素的生物可访问性和基因保护作用:黑色素及其生物活性之间的关系
Virginia Temiño1, Gisela Gerardi1, Monica Cavia-Saiz1
1Department of Biotechnology and Food Science, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos, 09001 Burgos, Spain.
Foods (Basel, Switzerland)
|September 9, 2023
概括
面包皮中的黑色素是生物可访问的,并且表现出抗氧化和复苏保护作用,没有毒性. 这些发现支持它们作为食品中的功能成分的使用.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 营养科学 营养科学
背景情况:
- 面包是一种面包制品的副产品,具有功能成分开发的潜力.
- 梅拉诺因是从梅拉德反应中衍生出来的,是棕色食物 (如面包皮) 中发现的化合物.
- 使用面包皮中的黑色素可以增加价值,并有助于健康益处.
研究的目的:
- 评估面包皮中的黑色素的生物可访问性,生物活性和复苏保护作用.
- 为了评估这些黑色素素素分量的安全性和肠道屏障运输.
- 探索面包皮黑色素在功能性食品开发中的潜力.
主要方法:
- 通过模拟胃,肠和结肠消化来评估黑色素的生物可访问性.
- 在Caco-2细胞上使用MTT和RTA试验评估了细胞毒性.
- 确定对Caco-2细胞单层的抗氧化活性和复苏保护作用.
主要成果:
- 生物可访问的黑色素在普通和软面包皮类型之间有所不同.
- 没有观察到细胞毒性;保持了膜完整性 (E-cadherin分布).
- 胃肠道和结肠分泌物表现出抗氧化活性,并穿过肠道屏障而不影响透性.
- 在Caco-2细胞中观察到显著的复苏保护作用.
结论:
- 来自面包皮的生物可访问的黑色素具有抗氧化和显著的复苏保护性质.
- 这些黑色素素被安全地通过肠道屏障运输,并表现出生物活性.
- 面包皮的黑色素素显示出作为功能性食品应用的有价值的生物活性化合物的前景.
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