低质酒含有可变的质和免疫原性表皮质含量
Mitchell G Nye-Wood1,2, Keren Byrne3, Sally Stockwell3
1School of Science, Edith Cowan University, Perth, WA 6027, Australia.
Foods (Basel, Switzerland)
|September 9, 2023
概括
无质酒可能对患有乳的人来说不安全. 艾丽莎测试可以错过发酵饮料中的质,导致一些酒的"无质"标签不准确.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 免疫学 免疫学 免疫学
背景情况:
- 无质饮食对于控制乳病和对质敏感性至关重要.
- 准确的质含量标签对于消费者安全至关重要,特别是在无质饮食的人群中.
- 目前关于无质声明的规定在不同司法管辖区之间存在差异,特别是对于酒等发酵产品.
研究的目的:
- 用质谱测量来调查无质酒中质检测的准确性.
- 为了比较ELISA和质谱检测结果,以检测发酵饮料中的质.
- 评估现有的无质法规对发酵食品的适用性.
主要方法:
- 液态染色体质谱法 (LC-MS) 用于检测质蛋白碎片和免疫原性表位.
- 与酶相关的免疫吸收试验 (ELISA) 用于进行质丰度比较测量.
- 进行了对照和据称无质酒的调查.
主要成果:
- 质谱检测检测到几个标记为无质的酒中的质.
- 一些"无质"酒比未经处理的对照酒含有更高的 (质蛋白) 水平.
- 发现ELISA的结果对于酒等发酵产品中的质检测可能不可靠.
结论:
- 在发酵饮料中,ELISA可能会产生质的虚假阴性.
- 由于测试方法的局限性,目前的无质法规可能无法充分保护患有乳病的个体.
- 质谱学提供了一种更可靠的方法来检测质在复杂的食物矩阵,如酒.
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