空气耐受性和多部位序列类型 Campylobacter jejuni 从商业肉加工厂分离出来
Diksha Pokhrel1, Hudson T Thames1, Li Zhang1
1Department of Poultry Science, Mississippi State University, Mississippi State, MS 39762, USA.
Foods (Basel, Switzerland)
|September 9, 2023
概括
导致腹的Campylobacter jejuni,在冷藏和冷的肉中显示了减少的数量. 超耐气菌株的减少更大,表明储存影响了细菌的存活率.
科学领域:
- 食品安全和微生物学
- 细菌病变和生存的发生.
- 禽肉加工污染的污染情况
背景情况:
- 坎比洛巴克特 (Campylobacter jejuni) 是美国细菌性腹的主要原因.
- 这种微气友性病原体在食品生产中持续存在,特别是家禽.
- 了解C. jejuni的耐风性对于监测和控制至关重要.
研究的目的:
- 评估C. jejuni从肉加工厂的耐风性和遗传多样性.
- 评估耐气性和储存条件对C. jejuni存活率的影响.
- 确定制冷和冷在减少C. jejuni种群中的有效性.
主要方法:
- 对40种C. jejuni分离物的空气耐受性分析 (对空气敏感,中等空气耐受性,超空气耐受性).
- 分离物的基因定型,以确定克隆复合体和序列类型.
- 随着时间的推移,在4°C和-20°C的温度下存储的肉鼓棍的生存研究.
主要成果:
- 62.5%的分离物是对空气敏感的,25%是中间的,12.5%是超空气耐受的.
- 隔离物属于4个克隆复合体 (CC) 和6个序列类型;CC-353占主导地位.
- 在4°C下7天 (0.40 log CFU/g) 和在-20°C下14天 (1.50 log CFU/g) 后,C. jejuni的数量显著下降.
- 在这两种储存条件下,高耐气菌株与空气敏感菌株和中间菌株相比,数量下降幅度更大.
结论:
- 制冷和冷有效地减少了肉鼓杆上的Campylobacter jejuni数量.
- 空气耐受性会影响冷藏期间C. jejuni的减少速度.
- 研究结果强调了冷链管理在控制家禽产品中C. jejuni污染方面的重要性.
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