一个多酶级联反应,用于对有机农药的色度识别,利用核心Pd@Pt纳米颗粒,具有高过氧化酶类活性
Zainabu Majid1, Qi Zhang1, Zhansen Yang1
1Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Foods (Basel, Switzerland)
|September 9, 2023
概括
一个新的色度传感器可以快速检测食品中的有机农药 (OPs). 这种使用Pd@Pt纳米颗粒的多酶级联系统为食品安全监测提供了快速,经济有效的解决方案.
科学领域:
- 农业化学 农业化学
- 纳米技术 纳米技术
- 分析化学 分析化学
背景情况:
- 有机农药 (OPs) 在农业中广泛使用,但由于乙胆酶 (AChE) 抑制,它们会给健康带来风险.
- 目前用于OP检测的方法往往是缓慢的,昂贵的,劳动密集型,需要改进的分析技术.
- 对食品中的OP残留物进行监测对于公众健康和环境安全至关重要.
研究的目的:
- 开发一种快速高效的色度传感器,用于量化食品基质中的有机农药 (OPs).
- 解决现有的OP检测方法的局限性,包括速度,成本和复杂性.
- 为了验证传感器的性能,检测一系列常见的OP.
主要方法:
- 采用了多酶级联系统,使用TMB作为基板和核心外Palladium@Platinum (Pd@Pt) 纳米粒子.
- Pd@Pt纳米颗粒的协同性能增强了用于OP识别的酶级联反应.
- 传感器的性能被评估为灵敏度,检测范围和定量各种OP的准确性.
主要成果:
- 开发的传感器成功量化了多个OP,包括Carbofuran,Malathion和Parathion,在各种度下.
- 基于Pd@Pt的级联方法证明了低检测极限 (1-2.5 ng/mL) 和大约50分钟的快速检测时间.
- 线性检测范围为10-200 ng/mL,R2值超过0.93,证实了传感器对农药残留物的定量准确性.
- 当传感器应用于真实食品样本时,它实现了超过80%的回收率.
结论:
- 基于多酶级联和Pd@Pt纳米颗粒的新型色度传感器为OP检测提供了一种简单,快速和有效的方法.
- 这种方法比传统方法有了显著的改进,提高了食品安全监测能力.
- 传感器的高灵敏度,速度和对食品样品的适用性凸显了其在常规分析农药残留物的潜力.
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