相关实验视频

Updated: Jul 16, 2025

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

组织RNA测序揭示了与术后状细胞复发相关的新生物标志物

Yanqiu Tang¹, Kehui Ren¹, Xufeng Yin¹

¹Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.

Journal of clinical medicine

|September 9, 2023

概括

研究人员确定了五个关键基因,这些基因可以预测手术后胆固醇的复发. 这一发现为早期检测和预防策略提供了潜在的生物标志物,改善了患者的治疗结果.

科学领域:

皮肤病学和免疫学研究.
分子生物学和基因表达分析.

背景情况:

状腺切除手术的复发率很高.
预测性生物标志物对于 keloid 复发是必要的.

研究的目的:

为了确定生物标志物来预测术后胆固醇复发.
为了探索反复发生的 keloids 中的免疫微环境.

主要方法:

从复发性和非复发性组的基路样本上进行RNA测序 (RNA-seq).
权重基因共同表达网络分析 (WGCNA) 来识别关键的基因模块.
分子复杂检测 (MCODE) 算法用于找到枢纽基因.
定量逆转录PCR (qRT-PCR) 和接收器操作特征 (ROC) 曲线分析.

主要成果:

五个枢纽基因 (CHI3L1,IL1RN,MMP7,TNFAIP3,TNFAIP6) 被确定为潜在的生物标志物.
这些基因在复发性 keloid 组显著上调 (p < 0.05).
组合基因的曲线下的面积 (AUC) 为0.776,表明预测值.
巨细胞,巨细胞和T细胞是 keloids 中的主要免疫成分.

结论:

鉴定到的五个基因显示出潜在的生物标志物,用于预测 keloid 复发.
这些发现提供了对预防 keloid 复发的遗传点的见解.
了解免疫微环境对于 keloid 管理至关重要.

关键词:

在RNA-Seqq.在WGCNA中,WGCNA是WGCNA.基因基因基因基因凯洛伊德 (Keloid) 是一种类型的物质.复发性复发性复发性手术手术手术手术手术手术手术

更多相关视频

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...