YBX1对HTLV-1转录的类效应
Susan Smith1, Jaideep Seth1, Amanda Midkiff1
1Center for Retrovirus Research, Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
International journal of molecular sciences
|September 9, 2023
概括
细胞转录因子YBX1与HBZ结合并激活HTLV-1基因表达. YBX1促进Tax介导的转录,但被HBZ抑制,影响ATL恶性瘤.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 人类T淋巴细胞病毒1型 (HTLV-1) 导致成人T细胞白血病/淋巴瘤 (ATL).
- 病毒基因Tax和HBZ对于HTLV-1的瘤发生和病变发生至关重要.
- 调节HTLV-1基因表达是其生命周期和疾病潜力的关键.
研究的目的:
- 研究细胞转录因子在HTLV-1基因调节中的作用.
- 为了确定病毒蛋白HBZ的结合伙伴.
- 阐明YBX1影响HTLV-1转录的机制.
主要方法:
- 酵母三混合查以确定HBZ结合伙伴.
- 西方涂抹和定量PCR用于评估蛋白质和转录水平.
- 在HTLV-1-转化细胞中,shRNA介导的YBX1的淘汰.
- 路西法雷斯记者测量测量以测量促销者活动.
- 染色体免疫沉以检测LTR关联.
主要成果:
- 酵母三混合物确定了Y盒结合蛋白1 (YBX1) 作为HBZ结合伙伴.
- YBX1激活了HTLV-1 5' LTR促进体的转录,并增强了Tax介导的转录.
- 敲除YBX1可以减少Tax和HBZ的表达,以及Tax与5'LTR的关联.
- YBX1与税收和HBZ互动.
- YBX1对5'LTR的激活被HBZ抑制,但被Tax增强.
- 在TLV-1感染细胞中,YBX1与5'和3'LTR相关.
结论:
- YBX1是一种细胞辅因子,通过5'和3'LTRs积极调节HTLV-1转录.
- YBX1促进了对5' LTR的税收招聘.
- HBZ对抗YBX1对5' LTR的转录激活.
- YBX1在HTLV-1基因表达和潜在的ATL病变发生过程中发挥着重要作用.
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