在衰老过程中对miR-146b表达的调节以及体育活动对其表达和原原体原始体的影响
Luca Dalle Carbonare1, Arianna Minoia1, Michele Braggio1
1Department of Engineering for Innovative Medicine, University of Verona, 37100 Verona, Italy.
International journal of molecular sciences
|September 9, 2023
概括
微RNA-146b-5p驱动介质干细胞分化,并且在斑马鱼和人类中随着年龄的增长而增加. 像走路这样的体力活动可以降低女性的这种微RNA,这表明它.
科学领域:
- 分子生物学分子生物学
- 衰老研究研究 衰老研究
- 干细胞生物学 干细胞生物学
背景情况:
- 微RNAs (miRNAs) 是关键的转录后调节者,与衰老和退行性疾病有关.
- miR-146b-5p与各种退行性疾病有关,但其在介质干细胞 (MSC) 承诺和衰老中的作用尚不清楚.
- 了解miRNA调制对于制定长寿策略和预防与年龄相关的疾病至关重要.
研究的目的:
- 在斑马鱼模型中研究介质细胞结合,分化和衰老过程中miR-146b-5p的调节.
- 分析不同年龄组的人体循环中的miR-146b-5p水平.
- 探索身体活动对miR-146b-5p水平和相关细胞过程的影响.
主要方法:
- 使用实时PCR (RT-PCR) 和西方斑点分析.
- 使用了细胞转染和三维 (3D) 培养技术.
- 在人类参与者身上测量了循环中的miR-146b-5p水平.
主要成果:
- miR-146b-5p表达促进MSCs的脂肪分化,并在斑马鱼成熟和衰老期间升高.
- 循环中的miR-146b-5p水平在女性中高于男性,并且与人类的衰老相关.
- 在女性中,步行降低了循环miR-146b-5p的调节,并增加了chondroprogenitors.
结论:
- miR-146b-5p作为一个重要的与年龄相关的生物标志物.
- 它可以作为一种有价值的标志物,用于识别干预措施,如身体活动,以打击与衰老相关的退化.
- 这项研究突出了促进健康衰老的潜在治疗目标.
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