相关实验视频

Updated: Jul 16, 2025

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation

Published on: March 24, 2023

在S.I.S. 皮肤上皮可以在肌素1-酸盐受体2-缺乏皮肤的过敏接触性皮肤炎中拯救皮肤

Kana Masuda-Kuroki¹, Shahrzad Alimohammadi¹, Anna Di Nardo¹

¹Department of Dermatology, School of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.

International journal of molecular sciences

|September 9, 2023

概括

斯塔菲洛科克斯 (Staphylococcus epidermidis) 的殖民增强了皮肤屏障功能,并防止过敏性接触性皮肤炎 (ACD),即使有斯芬戈辛-1-酸盐受体2 (S1PR2) 信号问题. 这一发现突显了S. epidermidis在皮肤健康方面的保护作用.

科学领域:

皮肤病学皮肤病学
免疫学免疫学免疫学
微生物学微生物学

背景情况:

氨酸-1-酸盐受体2 (S1PR2) 对于皮肤屏障的完整性至关重要.
在调节皮肤屏障方面,S1PR2和Staphylococcus epidermidis (S. epidermidis) 之间的相互作用尚未完全理解.

研究的目的:

研究S1PR2和S. epidermidis对皮肤屏障功能和过敏接触性皮肤炎 (ACD) 的联合作用.
阐明S. epidermidis介导的皮肤屏障改善背后的分子机制.

主要方法:

使用了S1pr2淘汰赛小鼠 (S1pr2^l) 和条件淘汰赛小鼠 (S1pr2^lK14-Cre).
使用3,4-Dibutoxy-3-cyclobutene-1,2-dione (SADBE) 诱导的ACD,并与S. epidermidis结合.
通过mRNA分析评估耳朵厚度,炎症标志物 (Il-1β,Cxcl2) 和紧结蛋白表达 (Claudin-1,Occludin).

主要成果:

在ACD期间,S. epidermidis的殖民减少了S1pr2^lK14-Cre小鼠的耳朵厚度,与S1pr2^l小鼠相比.
在S.epidermidis治疗的S1pr2^lK14-Cre小鼠中,炎症性基因表达 (Il-1β,Cxcl2) 较低.
在ACD期间,S. epidermidis殖民的S1pr2^lK14-Cre小鼠中,紧结蛋白 (Claudin-1,Occludin) 的表达上调.

结论:

S. epidermidis的殖民显著改善了皮肤屏障功能,并提供了对ACD的保护.
这种保护效应独立于S1PR2-介导的S1P信号传输,表明涉及其他途径.
S. epidermidis 是一种潜在的治疗点,用于治疗皮肤屏障疾病.

关键词:

3,4-二甲基-3-环丁-1,2- (SADBE) 是一种斯塔菲洛科克斯 (Staphylococcus epidermidis) 是一种表皮菌.接触性皮肤炎的情况斯芬戈-1-酸盐的使用方法斯芬戈-1-酸盐受体2 2 酸盐受体

更多相关视频

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model

Published on: July 13, 2009

Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

Published on: January 25, 2016

相关概念视频

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

Renewal of Skin Epidermal Stem Cells

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.