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基于TMT的蛋白质学分析衰老的核脉来自患有椎间盘退化症的患者

Guangzhi Zhang^1,2,3,4, Lei Li^1,2,3,4, Zhili Yang^1,2,3,4

¹Department of Orthopedics, Lanzhou University Second Hospital, Lanzhou 730000, China.

International journal of molecular sciences

|September 9, 2023

概括

细胞衰老在核脉动组织的细胞衰老有助于椎间盘退化 (IDD). 这项研究在衰老细胞中确定了301种差异表达的蛋白质,揭示了参与IDD进展的关键分子途径.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
整形外科整形外科整形外科

背景情况:

腰部疼痛是一个全球性的健康问题,其中40%的病例涉及椎间盘退化 (IDD).
核脉 (NP) 细胞衰老是IDD的关键驱动因素,但其分子机制尚未完全理解.

研究的目的:

为了研究IDD患者老化NP组织中的蛋白质变化.
确定参与IDD病变发生的关键蛋白质和途径.

主要方法:

陈旧和健康的NP组织的蛋白质组分析 (基于TMT) (n=3).
组织学染色 (H&E,Safranin O,Alcian蓝色,Masson) 和免疫组织化学.
生物信息学分析:基因本体学 (GO),基因和基因组的京都百科全书 (KEGG) 和蛋白质与蛋白质相互作用 (PPI).
针对特定蛋白质的免疫光验证.

主要成果:

在衰老的NP组织中鉴定出301种差异表达蛋白 (DEP) (92种上调,209种下调).
GO分析强调了NF-kappaB信号传递,细胞外区域和蛋白质修饰的参与.
凯格分析显示TGF-β,Wnt,IL-17和PI3K/Akt信号通路的丰富.
在老化的NP细胞中验证了MMP3,LUM,TIMP1和CDC42的表达.

结论:

这项研究阐明了IDD中NP细胞衰老的分子机制.
已识别的DEP为IDD治疗提供了潜在的治疗点.
研究结果提供了关于老化因素在IDD病原发生中的作用的见解.

关键词:

生物信息学分析分析椎间盘退化椎间盘退化它的核是脉冲的脉冲.蛋白质组学蛋白质组学双重质量标签 (Tandem Mass Tag) 是一个双重质量标签.

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