血小板丰富的等离子体通过Nrf2信号通路增加肌细胞的抗氧化防御
Alessia Tognoloni1, Desiree Bartolini2, Marco Pepe1
1Department of Veterinary Medicine, University of Perugia, 06126 Perugia, Italy.
International journal of molecular sciences
|September 9, 2023
概括
富含血小板血 (PRP) 通过减少损伤和细胞死亡来保护肌细胞免受氧化应激. 此外,PRP还激活抗氧化路径,促进肌的愈合和修复.
科学领域:
- 生物医学科学 生物医学科学
- 再生医学是一种再生医学.
- 运动医学 运动医学
背景情况:
- 肌病是普遍存在的,在马匹和人类运动员中造成残疾的疾病.
- 氧化应激 (OS) 和活性氧物种 (ROS) 涉及肌病的病因和肌修复障碍.
- 富含血小板血 (PRP) 是由于其生长因子和潜在的抗氧化作用而成为肌损伤的有前途的治疗方法.
研究的目的:
- 研究PRP对暴露于氧化应激的细胞的抗氧化作用.
- 为了确定PRP是否可以保护青细胞免受OS诱导的损伤和细胞死亡.
- 探索PRP在细胞中的抗氧化作用背后的分子机制.
主要方法:
- 主要的马类细胞被培养并暴露于诱导的氧化应激.
- 进行PRP治疗以评估其保护作用.
- 测量了氧化损伤标志物水平,细胞活力,Nrf2激活和抗氧化酶表达/活性.
主要成果:
- PRP显著降低了青细胞中蛋白质和脂质的氧化损伤.
- PRP保护了细胞细胞从氧化应激诱导的细胞死亡.
- PRP增加了核Nrf2水平,并诱导了抗氧化剂/II期排毒酶,包括SOD2,催化酶,HO-1,NQO1,GCLC和GST.
- PRP增强了catalase和谷氨S转移酶的酶活性.
结论:
- PRP表现出显著的抗氧化特性,保护肌细胞免受氧化应激.
- PRP激活细胞信号通路,特别是Nrf2通路,以恢复细胞平衡.
- 这些发现支持PRP作为促进肌再生和修复肌病变的治疗策略.
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