脂肪细胞衍生的近细胞因子调节了牛乳腺上皮细胞的体外发育
Żaneta Dzięgelewska-Sokołowska1, Alicja Majewska1, Adam Prostek1
1Department of Physiological Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159b, 02-776 Warsaw, Poland.
International journal of molecular sciences
|September 9, 2023
概括
牛乳腺上皮细胞 (bMEC) 的发育受到树皮细胞的影响. 前脂质细胞增强bMEC的迁移和氨酸的产生,而脂质细胞减少bMEC的亡并促进球形形成.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
背景情况:
- 乳腺流体,包括脂肪细胞和结缔组织,通过膜信号调节上皮细胞的发育.
- 对乳腺生物学来说,了解树皮-上皮相互作用至关重要.
研究的目的:
- 研究来自牛前脂质细胞和脂质细胞的膜因子对牛乳腺上皮细胞 (bMEC) 发育的影响.
- 评估在脂肪分化过程中分泌的因素如何影响bMEC活力,亡,迁移,球形形成和分泌活动.
主要方法:
- 使用了牛乳腺上皮细胞 (bMEC) 和牛脂肪基干细胞 (bASC) 的初级培养.
- bMEC从未分化的前脂肪细胞 (preA) 和不同分化阶段的脂肪细胞 (8,12,14天) 在条件介质中培养.
- 评估的参数包括细胞活力,细胞亡,迁移,Matrigel球形形成和alpha S1-casein分泌.
主要成果:
- 从差异化脂肪细胞 (12d,14d) 获得的条件介质减少了bMEC细胞亡和增加了球状体大小.
- 预脂细胞衍生条件介质增强了bMEC迁移.
- 前脂肪细胞因子在bMEC中刺激alpha S1-casein的产生,但仅在与益生菌一起治疗时.
结论:
- 前脂肪细胞和脂肪细胞分泌关键的膜因子,积极调节牛乳腺上皮细胞的发育.
- 干细胞在调节乳腺上皮细胞的行为和功能方面发挥着重要作用.
相关概念视频
01:58Cell Specific Gene Expression
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
01:15Receptor Downregulation in MVBs
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
01:12The Mammary Glands
The female breast is a hemispheric projection of variable size positioned anterior to the pectoralis major and serratus anterior muscles. A fascia layer composed of dense, irregular connective tissue connects it to these muscles.
Each breast features a pigmented projection known as the nipple, through which milk emerges via closely spaced openings of ducts, referred to as lactiferous ducts. Surrounding the nipple is a circular pigmented area of skin named the areola, which appears rough due to...
Each breast features a pigmented projection known as the nipple, through which milk emerges via closely spaced openings of ducts, referred to as lactiferous ducts. Surrounding the nipple is a circular pigmented area of skin named the areola, which appears rough due to...