唾液细胞外囊中的非编码RNA:Sjögren综合征诊断的新前沿?
Tanya Cross1, Kari Bente Foss Haug2, Berit Sletbakk Brusletto2
1The Regenerative Medicine Unit, Department of Medical Biochemistry, Oslo University Hospital, Ullevål, 0450 Oslo, Norway.
International journal of molecular sciences
|September 9, 2023
概括
研究人员在唾液细胞外囊 (EVs) 中确定了不同的RNA模式,用于诊断初级Sjögren综合征 (pSS). 这一发现突出了唾液EV-RNA作为一个有希望的,非侵入性的pSS检测生物标志物.
科学领域:
- 类风湿病学 类风湿病学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 肖格伦综合征是一种自身免疫性疾病,由于腺体炎症导致眼睛和口腔干燥.
- 目前对原发性Sjögren综合征 (pSS) 的诊断需要简化.
- 需要用于pSS的非侵入性诊断标志物.
研究的目的:
- 为了比较pSS患者和健康对照者之间的唾液细胞外囊泡 (EV) RNA含量.
- 为了确定潜在的非侵入性生物标志物用于pSS诊断.
- 探索微阵列技术在分析唾液EV-RNA中的实用性.
主要方法:
- 收集了来自11名pSS患者和11名对照组的刺激全唾液.
- 通过使用Qiagen exoRNeasy Midi Kit 来分离细胞外囊泡RNA (EV-RNA).
- 使用Affymetrix Clariom DTM微阵列和单向ANOVA分析了EV-RNA.
主要成果:
- 在所有样本中检测到9307个转录 (编码和非编码RNA).
- 在pSS和对照组之间确定了1475个统计学上显著的差异丰富的转录.
- 在pSS患者中观察到tRNA的下调,tRNA-Ile-AAT-2-1显示了2倍的差异.
结论:
- 唾液EV-RNA表现出明显的模式,使PSS患者与健康个体有所区别.
- 特定的tRNA降低调节表明它有可能成为pSS的新生物标志物.
- 唾液EV-RNA分析有望简化PSS诊断.
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