相关实验视频

Updated: Jul 16, 2025

A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa

A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa

Published on: June 27, 2025

根据大规模指纹和基因序列,区分和识别来自Populus的内生细菌

Xia Wang^1,2, Guanqi Wu^1,2, Shuo Han¹

¹College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China.

International journal of molecular sciences

|September 9, 2023

概括

矩阵辅助激光脱吸电离-飞行质谱时间 (MALDI-TOF MS) 有效地识别和分类来自Populus植物的内菌. 这种方法显示了类似于16S rRNA基因测序用于遗传学分析的结果.

科学领域:

微生物学微生物学
植物科学植物科学
生物技术是生物技术.

背景情况:

内生菌增强植物生长和抗压能力.
准确地描述这些细菌对于了解它们的益处至关重要.
矩阵辅助激光脱吸离离子飞行质谱时间 (MALDI-TOF MS) 是一种高通量细菌识别技术.

研究的目的:

使用MALDI-TOF MS和16S rRNA基因测序识别和分类来自各种Populus物种的内生细菌.
为了优化细菌采样和蛋白质质量指纹的准备.
为了比较MALDI-TOF MS的遗传学分析能力与16S rRNA基因测序.

主要方法:

从Populus的叶子,根和茎中分离出了内性细菌.
使用MALDI-TOF MS数据和16S rRNA基因测序进行了细菌鉴定和分类.
使用复合相关性指数 (CCI) 值和主要坐标分析 (PCoA) 分析了蛋白质质质量指纹.

主要成果:

优化的协议产生了有意义的蛋白质质量指纹.
CCI值表明细菌属和物种之间有足够的差异化.
使用MALDI-TOF多发性硬化形状的家族遗传学分析显示,与16S rRNA基因测序结果具有很高的相似性.

结论:

MALDI-TOF MS是一种可靠和有效的方法,用于区分和识别Populus中的内菌.
这项研究为使用蛋白质质量指纹进行细菌鉴定提供了宝贵的参考.
这种方法有助于理解内菌的生态作用.

关键词:

马尔迪-托夫 (MALDI-TOF) 是一个国家元首.不同化的差异化差异化.它们是内性细菌 (endophytic bacteria).预处理预处理相关性关联性关联性

更多相关视频

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Published on: May 5, 2023

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

相关概念视频

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Methods of Classification and Identification

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Differential Staining Technique

Differential Staining Technique

Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...