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分析前条件对淋巴细胞数量的影响
Undine Proschmann1, Puya Shalchi Amirkhiz1, Pauline Andres1
1Center of Clinical Neuroscience, Department of Neurology, University Hospital Carl Gustav Carus, University of Technology, 01307 Dresden, Germany.
International journal of molecular sciences
|September 9, 2023
概括
分析前的条件显著影响免疫细胞计数,特别是储存温度. 仔细考虑这些因素对于在临床实践中准确解释实验室结果至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 临床实验室科学 临床实验室科学
背景情况:
- 淋巴细胞是多发性硬化症 (MS) 病原体的核心.
- 针对淋巴细胞的免疫调节疗法对于MS治疗至关重要.
研究的目的:
- 调查分析前变量如何影响免疫细胞计数.
- 确定这些分析前效应对免疫细胞分析的临床相关性.
主要方法:
- 分析了来自20名健康捐献者的血液样本.
- 评估了储存温度,持续时间,门应用,身体位置和吸入力的影响.
- 免疫细胞频率使用多色流细胞计量量化.
主要成果:
- 在37°C保存24小时,显著降低了免疫细胞的数量.
- 围的持续时间,身体的位置和吸气力没有显著的影响.
- 储存温度成为影响细胞计数的分析前关键因素.
结论:
- 分析前的条件,特别是储存温度,显然会改变免疫细胞的数量.
- 免疫细胞亚群的实验室值需要仔细解释,考虑到分析前的变量.
- 标准化预分析程序对于可靠的诊断和研究结果至关重要.
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