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协同效应,改善细胞选择性,并阐明抗菌YS12的作用机制

Suzia Aktar Suchi¹, Dae Young Lee², Young Kyun Kim¹

¹Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea.

International journal of molecular sciences

|September 9, 2023

概括

新型抗微生物YS12,来自Bacillus velezensis,显示与抗生素的协同作用. 这种体表现出广泛的活性,低毒性和抗炎性质,使其成为有前途的治疗候选者.

科学领域:

微生物学微生物学
生物化学生物化学
免疫学免疫学免疫学

背景情况:

抗微生物 (AMP) 是作为常规抗生素的替代品被探索的.
之前的研究发现了YS12,一种来自Bacillus velezensis的新型AMP,具有广泛的抗菌活性.
新抗菌剂的需求是由抗生素耐药性增加所驱动的.

研究的目的:

评估YS12与现有抗生素结合的协同作用潜力.
调查YS12的抗菌疗效,安全性概况和作用机制.
评估YS12的抗炎性质.

主要方法:

分数抑制度指数 (FICI) 用于确定协同效应.
单独使用YS12,单独使用抗生素和组合使用的抗生素之间进行了细菌杀死动力学的比较.
在Raw 264.7细胞上进行了细胞毒性测试和血液溶解活性测试.
使用LPS结合试验,光测量和共聚焦显微镜研究了作用机制.
通过测量促炎介质 (TNF-α,IL-1β,NO) 来评估抗炎活性.

主要成果:

当与商业抗生素结合时,YS12表现出协同效应,导致更快的细菌杀死.
该在各种生理盐条件下表现出稳定性.
YS12对Raw 264.7细胞没有显著的细胞毒性和低血解活性.
机制研究显示,YS12通过静电相互作用与LPS结合,破坏细菌膜完整性,并在细胞质中定位.
YS12有效地降低了LPS诱导的促炎媒介,表明了抗炎潜力.

结论:

抗微生物YS12与抗生素具有显著的协同作用,并具有有利的安全性.
YS12的膜损伤机制和抗炎性质突显了其潜在的治疗价值.
YS12是开发新型治疗微生物感染和相关炎症的有希望的候选人.

关键词:

抗微生物 (AMP) 是一种抗微生物.细胞选择性细胞选择性分数抑制度指数 (FICI) 是指数.它们的作用机制.

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