在与谷氨复合和谷氨离合后以及谷氨离合后,血红蛋白特性发生变化
Iuliia D Kuleshova1, Pavel I Zaripov1,2, Yuri M Poluektov1
1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
International journal of molecular sciences
|September 9, 2023
概括
血球蛋白与谷氨结合非共价和共价. 非联结合对血红蛋白结构的影响比联联更为显著,影响红细胞的抗氧化保护.
科学领域:
- 生物化学 生物化学
- 转毒生物学 转毒生物学
- 蛋白质化学 蛋白质化学
背景情况:
- 血红蛋白对氧气运输至关重要,它与关键的细胞抗氧化剂谷氨相互作用.
- 这些相互作用,共价和非共价,影响血红蛋白的功能和红细胞的抗氧化防御.
- 甲基化血红蛋白作为氧化应激的标记物.
研究的目的:
- 描述氧血球蛋白与减少的谷氨 (GSH) 复合的特性.
- 描述谷氨基化血红蛋白 (通过SS键结合) 的特性.
- 为了比较与血红蛋白结合的非对应性与对应性谷氨的结构和功能效应.
主要方法:
- 圆形二重化谱光学 圆形二重化谱光学
- 拉曼光谱法 拉曼光谱法
- 红外光谱学是红外光谱学.
- 托芬的光效应是三的.
- 差分扫描光测量 差分扫描光测量
- 分子建模分子建模
主要成果:
- 协同质甲化改变了血红蛋白的二次结构 (降低了α-度),但没有改变它的血红蛋白环境,酸的光或热稳定性.
- 与GSH的非共价复合对二次结构的影响最小,但改变了血环境,托微环境,并降低了热稳定性.
- 与共价性谷氨酸化相比,非共价性复合体的形成对血红蛋白的三级和四级结构产生了更大的影响.
结论:
- 非性血红蛋白-谷氨相互作用显著影响蛋白质结构,可能会影响氧的亲和力和细胞抗氧化能力.
- 协同质甲化主要影响着血红蛋白的二次结构.
- 了解这些独特的相互作用对于解释谷甲化血红蛋白作为氧化应激标记物及其在细胞氧化还原恒温中的作用至关重要.
关键词:
拉曼散射是什么意思拉曼散射是什么意思一个圆形的二重化.差分扫描光测量 差分扫描光测量葡萄糖氨酸氨酸是什么?葡萄糖乙烯化 葡萄糖化血红蛋白 血红蛋白 是一个红外光谱学 红外光谱学分子建模分子建模托芬的光是因为光.更多相关视频
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