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嵌入和逆分散扫描电子显微镜 (EM-BSEM) 在与生物假体心脏有关的免疫类型比较优先

Alexander Kostyunin¹, Tatiana Glushkova¹, Elena Velikanova¹

¹Department of Experimental Medicine, Research Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Boulevard, Kemerovo 650002, Russia.

International journal of molecular sciences

|September 9, 2023

概括

化的大动脉保持细胞结构,而失败的生物假体心脏显示ECM降解和巨细胞透. 电子显微镜 (EM-BSEM) 提供了对生物假体心脏膜细胞性的优越分析.

科学领域:

心血管生物学心血管生物学
生物材料科学生物材料科学
细胞超结构细胞超结构

背景情况:

化大动脉 (AVs) 和失败的生物假体心脏 (BHVs) 分享调查方法,主要是免疫染色.
在区分细胞功能和预测BHV的结果方面,免疫抑制的限制是显而易见的.

研究的目的:

为了比较AVs和BHVs的细胞景观和细胞外矩阵 (ECM) 特性.
为了评估全样本非破坏性电子显微镜 (EM-BSEM) 分析膜细胞性的有效性.

主要方法:

使用多种免疫染色技术对AV和BHV进行比较分析.
全样本非破坏性电子显微镜 (EM-BSEM) 的应用,用于超结构分析.

主要成果:

AVs表现出明确的细胞层次结构,而BHVs表现出随机ECM降解和显著的巨细胞透 (≥80%白细胞).
BHVs具有多种过渡细胞表型 (例如CD45+CD31+,CD68+α-SMA+),与AVs中有限的细胞转化不同.
EM-BSEM成功地区分了巨细胞状态 (静止与矩阵降解),泡细胞和多核巨细胞,保持了组织完整性.

结论:

与AV相比,BHV的特点是积极的ECM降解和独特的细胞组成.
EM-BSEM是一种高级技术,用于详细的超结构分析和BHV内的细胞的功能评估,其性能优于传统的免疫染色.

关键词:

生物假肢心脏门石化性大动脉狭窄症甲状腺膜疾病甲状腺膜疾病细胞的可塑性细胞的可塑性内皮细胞到介质细胞的过渡.细胞外矩阵降解细胞外矩阵降解免疫表因型的确定巨细胞是什么?巨细胞是什么?结构退化结构退化膜间歇细胞的细胞.

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