人类角膜层中TGF-β1/-β3和激素受体之间的新相关性
Alexander J Choi1,2, Brenna S Hefley1,2, Sarah E Nicholas1,2
1North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
International journal of molecular sciences
|September 9, 2023
概括
转化生长因子β (TGF-β) 异型与3D角膜模型中的性激素受体相互作用. 这些相互作用在两性之间存在差异,影响着角膜流体生物学.
科学领域:
- 眼科医生 眼科 眼科
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
背景情况:
- 角膜的侧膜在维持其结构和功能方面发挥着至关重要的作用.
- 已知转化生长因子β (TGF-β) 异型和性激素会影响组织重塑和细胞反应.
- 了解TGF-β和角膜层中的性激素受体之间的相互作用对于了解眼睛健康和疾病至关重要.
研究的目的:
- 为了研究TGF-β1 (T1) 和TGF-β3 (T3) 与性激素受体之间的相互作用,在3D人类角膜纤维细胞模型中进行了研究.
- 确定性别作为生物变量 (SABV) 如何影响角膜层内的这些相互作用.
- 分析关键受体的表达,包括雄激素受体 (AR),孕激素受体 (PR),雌激素受体 (ERα,ERβ) 和淋巴激素受体.
主要方法:
- 原始人类角膜纤维细胞 (HCFs) 在3D透孔系统中培养了四周.
- 细胞补充了维生素C,并用TGF-β1或TGF-β3.3刺激细胞.
- 半定量西方斑点分析被用来评估各种性激素和淋巴激素受体的表达.
主要成果:
- 在女性结构中,TGF-β1显著上调了AR,PR,ERα,FSHR,GnRHR和KiSS1R.
- 在男性结构中,TGF-β1显著降低了FSHR和FSH-B的调节,同时上调了ERα,ERβ和GnRHR.
- 在女性结构中,TGF-β3显著上调PR,ERα,ERβ,LHR,FSHR和GnRHR,在男性结构中降低AR,ERα和FSHR.
结论:
- 这项研究表明,TGF-β异型和角膜层中的性激素受体之间存在显著的相互作用.
- 性作为一种生物变量,极大地影响了这些受体的表达模式.
- 需要进一步的研究,以充分阐明这些发现的信号机制和临床影响.
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