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生物材料表面特性对工程神经组织对脊髓再生的影响

Victor A da Silva¹, Bianca C Bobotis¹, Felipe F Correia¹

¹Laboratório de Neurogenética, Universidade Federal do ABC, Alameda da Universidade s/n, São Bernardo do Campo 09606-070, SP, Brazil.

International journal of molecular sciences

|September 9, 2023

概括

选择合适的生物材料是脊髓损伤 (SCI) 组织工程的关键. 聚 (ε-caprolactone) (PCL) 和聚 (L-乳酸) (PLLA) 支架最好支持神经细胞的附着和分化,用于SCI修复.

科学领域:

生物材料科学生物材料科学
神经科学是一个神经科学.
组织工程是组织工程.

背景情况:

组织工程旨在使用生物材料支架来修复脊髓损伤 (SCI).
不同的生物材料具有独特的特性,影响神经细胞的行为和分化.
了解细胞物质相互作用对于有效的SCI治疗策略至关重要.

研究的目的:

为了评估神经细胞在奇托 (CHI),多 (ε-caprolactone) (PCL) 和多 (L-乳酸) (PLLA) 支架上的行为.
确定生物材料表面特性对细胞形态,生存和分化的影响.
确定最佳的生物材料,以促进SCI中的神经再生.

主要方法:

在CHI,PCL和PLLA支架上播种神经原生细胞 (NPCdn,hiPSC-NPC) 和新生大鼠脊髓细胞 (SCC).
使用扫描电子显微镜,流细胞计和免疫光检测评估细胞附着,形态和分化.
计算分析用于研究细胞物质表面相互作用.

主要成果:

生物材料的表面特性显著影响了细胞形态和分化.
PCL和PLLA支架支持SCC和NPCdn的附着和成熟,与CHI不同.
虽然所有材料都没有表现出细胞毒性,但PCL和PLLA在SCC中专门引导神经元分化和网络形成.
人类诱导的多能干细胞衍生神经原生细胞 (hiPSC-NPC) 在所有测试的生物材料上都显示出质分化增加.

结论:

生物材料的选择对于成功的脊髓损伤组织工程至关重要.
与基多相比,PCL和PLLA在支持神经细胞生长和分化方面显示出更高的潜力.
对PCL和PLLA支架的进一步研究可以推进SCI再生的治疗策略.

关键词:

生物材料的表面是生物材料.替代细胞的替代细胞这就是hiPSCs.人类诱导的多能干细胞干细胞.脊髓损伤导致的脊髓损伤组织疗法是组织疗法.

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