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Updated: Jul 16, 2025

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
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在dUTP C5-替代剂和DNA聚合酶之间非共价相互作用降低PCR效率

Olga A Zasedateleva1, Sergey A Surzhikov1, Viktoriya E Kuznetsova1

  • 1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991 Moscow, Russia.

International journal of molecular sciences
|September 9, 2023
PubMed
概括

分子建模和PCR实验显示,修改后的dUTPs (脱氧氨酸三酸盐) 降低了PCR放大效率. 这种减少与dUTP替代剂和DNA聚合酶之间的非共价键增加有关.

关键词:
A和B家族的DNA聚合酶.修改了C5的dUTPs可以使用.进行PCR放大.在X射线中,X射线结构结构是什么?分子建模分子建模非共价相互作用非共价相互作用

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