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甲类HLA表达与DNA损伤和免疫细胞透到性结肠炎中的失塑性和新形成性病变有关
Haruka Okami1, Naoya Ozawa1, Makoto Sohda1
1Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi 371-8510, Japan.
International journal of molecular sciences
|September 9, 2023
概括
人类白细胞抗原I类 (HLA-I) 表达和DNA损伤在性结肠炎 (UC) 和结肠癌 (CC) 中升高. 向DNA损伤可能会影响UC的免疫反应和致癌.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 胃肠病学 胃肠病学
背景情况:
- 性结肠炎 (UC) 与人类白细胞抗原I类 (HLA-I) 作为遗传因素有关.
- 了解与UC相关的发育不良/结肠直肠癌 (CC) 和零星结肠直肠癌 (SCRC) 的DNA损伤和免疫细胞透是至关重要的.
研究的目的:
- 调查DNA损伤,HLA-I表达和PD-L1在生殖不良/CC和SCRC中的作用.
- 分析与这些标记物相关的免疫细胞透 (CD8,FOXP3,CD68,CD163).
主要方法:
- 在SCRC,UC和相邻的正常粘膜 (NM) 的组织样本上进行了免疫组织化学染色.
- 分析的标记物包括HLA-I,PD-L1,γH2AX (DNA损伤),CD8,FOXP3,CD68和CD163.3等.
主要成果:
- 脑膜硬化和发育不良/脑膜硬化组织的膜HLA-I阳性上皮细胞显著高于NM和SCRC.
- 高HLA-I表达在发育不良/CC与增加的γH2AX和PD-L1阳性相关.
- 与UC和SCRC相比,HLA-I高形/CC表现出CD8+T细胞和CD68+巨细胞的透率更高.
结论:
- 缺血症/CC中的DNA损伤与高HLA-I表达和显著的免疫细胞透有关.
- 在UC中调节DNA损伤可能会通过HLA-I抗原呈现影响免疫检查点蛋白和致癌.
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