福克斯O3调节神经干细胞中的循环节律
Swip Draijer1, Raissa Timmerman1, Jesse Pannekeet1
1Swammerdam Institute of Life Sciences, University of Amsterdam, 1018 WB Amsterdam, The Netherlands.
International journal of molecular sciences
|September 9, 2023
概括
转录因子FoxO3通过调节时钟基因表达来破坏神经干细胞中的昼夜节律. 失去FoxO3还会延迟海马中神经干细胞增殖的时间.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 时间生物学 时间生物学
背景情况:
- FoxO转录因子和昼夜时钟调节新陈代谢,细胞循环和干细胞平衡.
- 众所周知,FoxO3可以保护成年神经干细胞并调节肝脏的昼夜节律.
- FoxO3在神经干细胞昼夜节律中的作用以前是未知的.
研究的目的:
- 为了调查FoxO3是否调节神经干细胞中的昼夜节律.
- 确定FoxO3影响神经干细胞昼夜节律的机制.
- 为了检查FoxO3缺乏对神经干细胞增殖时间 in vivo的影响.
主要方法:
- 缺少FoxO3.3的神经干细胞培养物中昼夜节律的破坏.
- 在单细胞水平上评估昼夜节律,使用Rev-Erbα-VNP报告员.
- 关于昼夜时钟组件与FoxO3.3共同占用的已公布数据的元分析.
- 在FoxO3缺乏的小鼠中分析海马神经干细胞的增殖.
主要成果:
- 失去FoxO3通过调节时钟转录水平,扰乱神经干细胞培养中的昼夜节律.
- 在没有FoxO3.3的情况下,循环节律在单细胞水平上保持.
- 分析证实,FoxO3是一种时钟控制的基因,由多个昼夜组成部分共同占用.
- 在小鼠中,FoxO3缺乏会延迟海马神经干细胞增殖的昼夜阶段.
结论:
- FoxO3在神经干细胞内调节昼夜节律方面发挥着至关重要的作用.
- FoxO3 影响神经干细胞增殖的时间,影响整体平衡.
- 这些发现确立了FoxO3作为神经干细胞生物学昼夜调节的关键组成部分.
相关概念视频
02:19Circadian Rhythms and Gene Regulation
4.1K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.1K
01:26Stem Cell Niche
5.2K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.2K
01:01Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
01:37Neural Regulation
39.5K
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
39.5K
01:10Zygotic Development And Stem Cell Formation
5.2K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
5.2K
00:58Embryonic Stem Cells
28.7K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
28.7K