端粒的替代延长在狗囊细胞肉瘤中是罕见的
Theresa Kreilmeier-Berger1, Heike Aupperle-Lellbach2, Martin Reifinger3
1Department for Companion Animals and Horses, University of Veterinary Medicine, 1210 Vienna, Austria.
Cancers
|September 9, 2023
概括
端粒的替代延长 (ALT) 是一种癌症机制. 这项研究仅在4%的犬类囊性肉瘤中发现了低ALT活性,这表明它不是一个常见的治疗点,但可能具有诊断价值.
科学领域:
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
- 兽医医学 兽医医学 兽医医学
背景情况:
- 癌细胞利用端粒维护机制 (TMM) 逃避衰老,使得TMM成为治疗点.
- 端粒的替代延长 (ALT) 是人类肉瘤中常见的一种端粒酶独立的TMM.
- 囊性肉瘤 (HS) 是一种具有未知的ALT患病率的侵袭性癌症; 狗的HS可以作为一个可比的模型.
研究的目的:
- 用伯尔尼山狗 (BMD) 作为人类患者的模型,确定犬类囊性肉瘤 (HS) 中ALT的频率.
- 在狗HS样本中评估ALT活性和端粒含量 (TC).
- 评估ALT作为HS治疗标或诊断标记物的潜力.
主要方法:
- 对63只患有HS的狗 (47只骨髓瘤) 的甲固定型氨酸嵌入性瘤样本的回顾性分析.
- 使用放射标记C圆测定 (CCA) 评估ALT活动.
- 通过FISH验证ALT阳性,并评估相对端粒含量 (TC).
主要成果:
- 低ALT活性 (1-14%) 在47只 (4%) 的伯尼山狗中被检测到2只患有HS.
- 所有其他犬类HS样本均为ALT阴性.
- 超明亮的端粒焦点证实了ALT阳性;端粒含量与ALT活性没有相关性.
结论:
- 替代性端粒延长 (ALT) 在狗囊性肉瘤 (HS) 中不常见.
- 因此,ALT不太可能成为HS的主要治疗点.
- 存在ALT可能在犬类HS中具有诊断意义.
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