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MiR-148a-3p通过向SLC7A1111来促进结直肠癌细胞铁亡

Elisa Martino¹, Anna Balestrieri², Francesca Aragona³

¹Department of Precision Medicine, University of Campania Luigi Vanvitelli, Via L. De Crecchio 7, 80138 Naples, Italy.

|September 9, 2023

概括

微RNA 148a-3p通过抑制细胞活力和诱导铁亡,在结直肠癌 (CRC) 中起到瘤抑制作用. 准miR-148a-3p/SLC7A11通路为CRC提供了新的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
细胞死亡机制细胞死亡机制

背景情况:

铁,一种依赖于铁的细胞死亡,以及微RNA (miRNA) 失调都与结直肠癌 (CRC) 的进展有关.
在各种癌症中,miR-148a-3p的瘤抑制作用已经确立,但其在CRC中的功能仍然不清楚.

研究的目的:

在结直肠癌细胞死亡中研究miR-148a-3p的分子机制和调控标.
确定miR-148a-3p是否可以作为CRC中的瘤抑制剂.

主要方法:

定量实时聚合酶链反应 (qRT-PCR) 来评估CRC细胞和正常结肠细胞中的miR-148a-3p表达.
在CRC细胞中过度表达miR-148a-3p,以评估其对细胞活力,细胞亡,铁亡标志物和SLC7A11表达的影响.
生物信息分析用于预测miR-148a-3p目标.

主要成果:

与正常细胞相比,CRC细胞 (SW480,SW620) 的miR-148a-3p表达显著减少.
miR-148a-3p的过度表达抑制了CRC细胞活力,诱导了细胞亡,促进了铁亡 (由脂质过氧化,GPX4下调,铁积累证明),并抑制了SLC7A11的表达.
降低miR-148a-3p的调节增加了SLC7A11的表达,并减少了铁亡.

结论:

miR-148a-3p通过向SLC7A11并诱导铁亡,在结直肠癌中起到瘤抑制作用.
在结直肠癌治疗中,miR-148a-3p/SLC7A11通路是潜在的治疗标.

关键词:

在 SLC7A1111 中.结肠直肠癌是什么意思铁性化 (ferroptosis) 是一种脂质过氧化过氧化在 miR-148a-3p 中使用.

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