相关实验视频

Updated: Jun 11, 2026

A Method for Targeted 16S Sequencing of Human Milk Samples

A Method for Targeted 16S Sequencing of Human Milk Samples

Published on: March 23, 2018

在产后2周对与哺乳期相匹配的人类和牛奶样本进行非向的代谢分析

Dominick J Lemas^1,2,3, Xinsong Du¹, Bethany Dado-Senn⁴

¹Department of Health Outcomes and Biomedical Informatics, College of Medicine, University of Florida, Gainesville, FL 32608, USA.

|September 9, 2023

概括

牛乳通常用于婴儿,但可能会导致不良结果. 这项研究比较了人类和牛奶代谢物,发现超过80%的重叠和在微生物代谢中起着重要的作用,影响婴儿健康.

科学领域:

营养生物化学营养生物化学
婴儿健康婴儿健康
代谢学代谢学代谢学

背景情况:

在婴儿饮食中,牛奶经常取代母乳.
这种替代与潜在的不良婴儿健康结果有关.
了解牛奶成分的差异对于婴儿营养至关重要.

研究的目的:

为了比较人类和牛奶在分娩后两周的代谢.
确定影响婴儿健康的独特和共同的代谢物.
探索牛奶代谢物在微生物代谢和宿主相互作用中的作用.

主要方法:

在分娩后两周收集人乳和牛乳样本 (每个n=10).
使用非定位的高分辨率液态色谱-并联质谱 (HR LC-MS/MS).
使用MS-DIAL和MS-FLO处理和注释的新陈代谢数据.

主要成果:

在早期哺乳期,超过80%的牛奶代谢在人乳和牛乳之间共享.
近80%的牛奶代谢物可能会影响微生物代谢.
显著的重叠表明在婴儿肠道微生物组发育中具有共同的功能作用.

结论:

非定位代谢学是有效的识别共享和独特的牛奶代谢物.
牛奶代谢产物在微生物代谢和微生物与宿主相互作用中发挥着重要作用.
需要进一步的研究来阐明特定代谢物对婴儿健康结果的影响.

关键词:

母乳养哺乳期母乳养人类母乳是人类的母乳.婴儿养婴儿养代谢生物组的代谢生物组

更多相关视频

An Efficient Single—Person Technique for Milk Sampling from Laboratory Mice

An Efficient Single—Person Technique for Milk Sampling from Laboratory Mice

Published on: March 28, 2025

Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings

Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings

Published on: February 7, 2025

相关概念视频

Development of Human Microbiota

Development of Human Microbiota

The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from the skin...