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一种新的冷适应性和高性酸酸酶,具有酸寡糖制剂的潜力

Hai-Ying Wang¹, Zhi-Fang Chen^1,2,3, Zhi-Hong Zheng^1,4

¹Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.

Molecules (Basel, Switzerland)

|September 9, 2023

概括

一种新型的酸盐酶,Alyw208,是为工业用途设计的,显示出增强的活性和稳定性. 这种酶有效地产生酸寡糖化物 (AO),具有各种应用的理想性质.

科学领域:

生物技术和酶学生物技术和酶学
海洋天然产品海洋天然产品
食品科学食品科学食品科学

背景情况:

酸寡糖 (AO) 具有多样化的生物活性,但它们的产生受到可用的酸酶的特性所限制.
商业酸盐酸酶通常具有低活性,低热稳定性和有限的离子耐受性,阻碍了工业应用.
需要强大的酸盐酸酶,适合大规模生产AOs.

研究的目的:

为了表达和描述来自*Vibrio* sp.的酸酶Alyw208的特征. 在食品级宿主 (*Yarrowia lipolytica*) 中的W2.
评估Alyw208在工业应用中的适用性.
评估Alyw208在生产酸盐寡糖的潜力.

主要方法:

在Yarrowia lipolytica*中,阿尔金酸盐酶Alyw208的重组表达.
Alyw208的生物化学表征,包括最佳温度,pH值和盐分耐受性.
对降解产品 (AO) 和其聚合度 (DP) 的分析.

主要成果:

Alyw208在35°C和pH10.0处表现出最佳活性,在10-55°C之间保持显著活性.
酶活性被1.5M NaCl增强了大约2.1倍,表明良好的盐耐受性.
Alyw208是一种双功能的内解性酸酶,有效地将多M和多G酸分解为AO (DP2-6) 和单体 (DP1-6).

结论:

标志性的酸盐酶Alyw208表现出有利的适应寒冷和性质,以及良好的热稳定性和盐分耐受性.
Alyw208的双功能性和高效降解能力使其成为生物技术和工业生产酸寡糖的有希望的候选者.
这种工程酶解决了当前酸盐酶的局限性,为更广泛的AO应用铺平了道路.

关键词:

雅罗维亚 (Yarrowia lipolytica) 是一个高脂的植物.阿尔基酸酸酶的使用酸的寡糖化物适应寒冷的适应者高性的高性含量

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