核桃核油和脱脂提取物增强介质干细胞干细胞和延迟衰老
Marwa A Elsied1, Zeina W Sharawi2, Hadba Al-Amrah2
1Anatomy and Embryology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
Molecules (Basel, Switzerland)
|September 9, 2023
概括
核桃提取物可以增强间酶干细胞 (MSC) 的干度,并减少衰老. 脱脂核桃提取物显示出更大的益处,突出了其在再生医学应用中的潜力.
科学领域:
- 干细胞生物学 干细胞生物学
- 生物化学 生物化学
- 营养保健品 营养保健品
背景情况:
- 介质干细胞 (MSC) 对再生医学至关重要,但由于干细胞的减少和衰老的增加而受到限制.
- 开发化合物来增强MSC茎度和延迟衰老对于治疗应用至关重要.
- 核桃核油 (WKO) 和脱脂核桃 (WKD) 提取物被探索它们对MSCs的潜在影响.
研究的目的:
- 调查WKO和WKD提取物对骨髓 (BM) -MSCs的干和衰老的影响.
- 评估这些提取物对BM-MSCs诱导衰老和炎症的保护作用.
- 为了比较WKO和WKD提取物在调节MSC属性的有效性.
主要方法:
- 用H2O2和LPS治疗BM-MSC,以诱导衰老和炎症.
- 用HPLC对WKO和WKD提取物进行了植物化学分析.
- 评估了干细胞 (增殖,迁移),衰老标志物 (p53,p21,SIRT1,AMPK),氧化应激,抗氧化活性,炎症性细胞因子和细胞周期.
- 检测包括MTT,qPCR,ELISA和流细胞计.
主要成果:
- WKO和WKD提取物增强了BM-MSC干性,增加了生命力,减少了细胞亡,上调MMP9,下调TIMP1,并促进G0/G1细胞周期停止.
- 这两种提取物通过降低p53/p21,增加SIRT1/AMPK,改善抗氧化能力 (减少ROS,增加CAT,SOD,GPx,Nrf2,HO-1),降低炎症类细胞因子 (TNFα,IL1β,NF-κB) 来降低BM-MSC衰老.
- 与WKO提取物相比,WKD提取物表现出优越的效果,这归因于更高的黄和含量和更强的抗氧化活性.
结论:
- WKO和WKD提取物有效地增强了大鼠BM-MSC的干度,并防止衰老.
- 脱脂核桃提取物 (WKD) 由于其增强的抗氧化特性,显示出更大的潜力.
- 这些核桃提取物有望提高MSCs在再生医学中的治疗疗效.
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