叶片的应用,以减少咖啡苗的诱导干燥应激效应:诱导原始化或缓解效应?
Gustavo Ferreira de Sousa1, Maila Adriely Silva1, Mariana Rocha de Carvalho2
1Soil Science Department, Federal University of Lavras, Lavras 37200-000, MG, Brazil.
Plants (Basel, Switzerland)
|September 9, 2023
概括
在透应激之前应用 (Se) 通过增强抗氧化酶来改善咖啡苗的耐受性. 在压力后应用Se可能会对植物的水潜力产生负面影响.
科学领域:
- 植物生理学 植物生理学
- 农业科学 农业科学
- 环境科学 环境科学
背景情况:
- 由PEG-6000等剂引起的透应激会对咖啡苗的生长和生理功能产生负面影响.
- 酶活动,如谷氨缩小酶 (GR) 和超氧化脱酶 (SOD),是植物应激反应的关键指标.
- 众所周知, (Se) 在植物防御机制中起到作用,抵御各种压力.
研究的目的:
- 为了确定 (Se) 供应在增强咖啡幼苗中透应激耐受性的有效性.
- 确定叶子Se应用的最佳时间,以减轻透应激的不良影响.
- 评估Se对关键酶活性和压力条件下的水潜力的影响.
主要方法:
- 咖啡幼苗使用聚乙烯甘醇-6000 (PEG-6000) 进行了透应激.
- 与施加压力相比,Se的叶片应用是在五个不同的时间点进行的.
- 在不同的处理条件下测量了酶活性 (GR,SOD,CAT,APX) 和植物水潜力 (ψw).
主要成果:
- 透应激诱导轻度应激 (ψw -1.5MPa至 -2.5 MPa) 并显著降低了对照工厂的GR和SOD活动.
- (Se) 的叶子供应,特别是在压力发生前8天,大大增加了CAT,APX和SOD酶的活性.
- 压力后的Se应用似乎加剧了压力,导致植物水潜在的减少.
结论:
- 在透应激之前对叶子施加Se比应激后的应用更有效地提高了咖啡苗的耐受性.
- 早期的Se应用增强了关键的抗氧化酶活动,增强了植物对透应激的防御能力.
- 在透应激后应用Se可能会给植物带来额外的压力,从而对植物水的状态产生负面影响.
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