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在玻璃中转化咖啡菌种. 在SETISTM生物反应器系统中的体质胚胎

Hugo A Méndez-Hernández¹, Rosa M Galaz-Ávalos¹, Ana O Quintana-Escobar¹

¹Plant Biochemistry and Molecular Biology Unit, Yucatan Scientific Research Center, Street 43, No.130 x 32 y 34, Mérida 97205, Yucatán, Mexico.

Plants (Basel, Switzerland)

|September 9, 2023

概括

在咖啡 (Coffea spp.) 中体胚生成 (SE). 使用SETISTM生物反应器进行了优化,以实现高效,大规模的植物传播. 这种方法产生了超过90%的适应度的强壮植物,为商业咖啡生产提供了具有成本效益的解决方案.

科学领域:

植物生物技术植物生物技术
农业科学农业科学
园艺园艺园艺园艺

背景情况:

实体胚生成 (SE) 对于大量植物的繁殖至关重要,特别是对于像咖啡 (Coffea spp. ) 的情况.
现有的咖啡的SE方法提供了基因变异性和低索马克隆变异性,使它们成为生产农业强壮植物的理想选择.
成功的大规模传播依赖于集成,优化和可转移的系统技术.

研究的目的:

开发一种高效,具有成本效益的咖啡 (Coffea spp.) 试管繁殖系统. 使用体质胚胎生成.
将两个成功的SE系统与SETISTM生物反应器浸泡系统集成,用于体质胚胎发育.
评估生物反应器再生咖啡植物的生长,活力和适应.

主要方法:

用直接和间接的SE协议对Coffea canephora和Coffea arabica获得体质胚胎.
将卵胚胎转移到SETISTM生物反应器中,用于体外发育成幼苗.
评估植物参数,包括新鲜体重,长度,叶子和根部发育,口腔指数,相对含水量和体外生存率.

主要成果:

为Coffea spp.开发了一个具有成本效益的SE系统. 使用SETISTM生物反应器,在十周后产生完整而有力的植物.
优化了花园土壤,泥炭和农矿石 (1:1:0.5比) 的基板组合,以适应幼苗的适应.
在生物反应器再生的咖啡苗中实现了超过90%的适应率.

结论:

将SE与SETISTM生物反应器集成,为咖啡植物的商业化体外繁殖提供了一种高效的技术.
该协议作为扩大其他农业经济重要物种繁殖的基础.
需要进一步的研究,以解决其他生产链和现场层面的挑战,以实现成功的商业化.

关键词:

阿拉伯咖啡是阿拉伯咖啡.咖啡 (Coffea canephora) 是一种咖啡.适应和适应的过程生物反应器是一个生物反应器.微传播是一种微传播.扩大规模,扩大规模.实体胚胎发生体质胚胎发生.

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