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发现MbIAA19的一个新功能可以调节植物矮体生长的马卢斯

Jian Wang¹, Li Xue¹, Xiao Zhang¹

¹College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.

Plants (Basel, Switzerland)

|September 9, 2023

概括

果树的矮化与MbIAA19基因有关,该基因通过影响auxin水平来调节植物的高度和内部节点长度. 这一发现为控制果树架构提供了洞察力.

科学领域:

植物科学植物科学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

果树的架构,以植物高度和内部节点长度为特征,对于评估矮根茎至关重要.
植物激素是果树中植物高度和内部节点长度的主要调节者.

研究的目的:

为了研究对自然存在的Malus baccata矮突变物 (Dwf) 中的矮化负责的遗传机制.
为了确定关键的基因和途径参与调节矮化和激素信号传递.

主要方法:

使用RNA-seq对野生类型 (WT) 和DWF突变Malus baccata进行比较转录组分析.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富分析.
内源性英多尔-3-酸 (IAA) 水平的量化和遗传转化试验.

主要成果:

差异基因表达分析揭示了与激素信号传递相关的多个差异表达基因 (DEGs),特别是辅酶通路.
发现Aux/IAA转录因子基因MbIAA19在DWf突变中由于促进体突变而被下调.
与WT相比,在DWf突变体中观察到较低的内源性IAA水平. 针对MbIAA19的RNA干扰 (RNAi) 导致植物高度,内部节点长度和IAA水平降低.

结论:

MbIAA19在调节化和内源性IAA水平方面发挥着重要作用.
鉴定的遗传机制为理解和潜在地操纵果树架构提供了基础.

关键词:

在Aux/IAAA中工作.马卢斯·巴卡塔塔 (Malus baccata) 是一个小鱼类.辅助剂辅助剂辅助剂一个矮人矮人.转录组 (transcriptome) 是一个转录组.

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