相关实验视频

Updated: Jul 16, 2025

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
10:18

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Published on: August 20, 2011

24.4K

在茶叶植物 (Camellia sinensis) 中病毒诱导的基因沉默

Wei Yang1, Xianya Chen1, Jiahao Chen1

  • 1College of Horticulture, South China Agricultural University, Guangzhou 510642, China.

Plants (Basel, Switzerland)
|September 9, 2023
PubMed
概括

病毒诱导的基因沉默 (VIGS) 为茶叶植物的基因功能验证提供了一种有效的方法. 皮皮球注射促进了有效的基因沉默,使得Camellia sinensis的快速功能分析成为可能.

关键词:
卡梅利亚·辛内西斯 (Camellia sinensis) 是一种植物.植物脱酶 (PDS) 是一种烟草公牛病毒 (TRV)病毒诱导的基因沉默是病毒引起的.

更多相关视频

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato06:34

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato

Published on: June 10, 2009

52.9K
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance08:17

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

Published on: August 23, 2013

17.1K

相关概念视频

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.8K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K