基于DNA链位移的小分子分析,使用细菌荷多普辛光电转换器:以ATP为例
Hsiu-Mei Chen1, Wen-Chang Wang2, Hong-Ren Chen1
1Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan.
Sensors (Basel, Switzerland)
|September 9, 2023
概括
这项研究开发了一种使用紫色膜 (PM) 的新型生物传感器,用于敏感检测腺三酸盐 (ATP) 和细菌. 基于PM的传感器提供了对小分子和微生物群体的快速,选择性和定量分析.
科学领域:
- 生物光子学 生物光子学
- 生物传感器技术技术
- 分子诊断学 分子诊断学
背景情况:
- 紫色膜 (PM) 带有光活性后体素是一种敏感的光电转换器.
- 现有的PM应用侧重于蛋白质和微生物;将扩展到小分子进行探索.
研究的目的:
- 开发一种基于PM的生物传感器,用于对小分子进行定量检测,以三氨酸腺酸盐 (ATP) 为模型.
- 为了适应PM生物传感器用于细菌群体分析.
主要方法:
- 使用黄金纳米粒子 (AuNP) 标记和DNA链位移的反向检测策略被采用.
- 一个PM单层电极用一种ATP特异性体功能化,并与一种AuNP标记的DNA链混合.
- 用于定量分析,通过ATP结合测量了光电流调制.
主要成果:
- 生物传感器在15分钟内实现了直接的单步ATP检测.
- 对ATP的检测极限低至5nM,动态范围为5nM0.1mM.
- 该传感器表现出高选择性,稳定性,并在低CFU/mL水平下成功量化了细菌和大肠杆菌.
结论:
- 开发的基于PM的生物传感器有效量化了像ATP这样的小分子.
- 这项技术可以同时量化各种小分子和微生物检测.
- 基于PM的生物传感器代表了各种定量测试的多功能平台.
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