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局部低频振动加速了高血糖大鼠模型中全厚伤口的愈合
Daijiro Haba1,2, Qi Qin2, Chihiro Takizawa2
1Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Journal of diabetes investigation
|September 9, 2023
概括
局部低频振动 (LLFV) 疗法通过改善血液流动和减少炎症,加速糖尿病大鼠的伤口愈合. 这种非侵入性治疗增强了糖代谢,促进了高血糖伤口的更快恢复.
科学领域:
- 生物医学工程 生物医学工程
- 再生医学是一种再生医学.
- 糖尿病并发症 研究 研究 糖尿病并发症
背景情况:
- 糖尿病足 (DFU) 由于慢性高血糖和炎症而表现出受损的愈合,阻碍了血管扩张和血管生成.
- 众所周知,局部低频振动 (LLFV) 促进血管扩张和血液流动,但其在糖尿病疾病中的有效性受到高血糖和炎症的限制.
研究的目的:
- 研究LLFV对高血糖大鼠的伤口愈合的影响.
- 确定LLFV是否可以改善糖代谢,减少炎症,增强糖尿病伤口中的血管扩张和血管生成.
主要方法:
- 带有实验诱导的伤口的斯特雷普佐托辛诱导的高血糖斯普拉格 - 达利大鼠从伤口后的1-14天开始每天40分钟用LLFV (50Hz,0-1,500mVpp) 治疗.
- 评估了伤口愈合,糖代谢标志物 (Glo-1,Slc2A4,甲基),炎症标志物 (Tnfa,Ptx3,Ccl2,M1/M2巨细胞比率) 和血管生成因子 (Nos3,Vegfa).
主要成果:
- 在600和1000mVpp的LLFV显著减少了受伤后5-7天的相对伤口面积.
- LLFV调节了糖代谢和炎症,由改变的Glo-1,Slc2A4,Tnfa,Ptx3,Ccl2表达和降低M1/M2巨细胞比率证明.
- 通过LLFV治疗 (1000mVpp) 观察到增强的血管扩张和血管生成,在创伤后的第14天,由上调的Nos3和Vegfa表达表示.
结论:
- 在高血糖条件下,LLFV显示了促进伤口愈合的治疗潜力.
- 实际上,LLFV有效地改善了糖代谢,减少了炎症,从而增强了糖尿病伤口中的血管扩张和血管生成.
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