野生类型和转基因Pichia pastoris的生长和基质消耗的宏观建模
Jewel Ann Joseph1, Simen Akkermans1, Jan F Van Impe1
1BioTeC+, Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Ghent, Belgium.
Biotechnology journal
|September 9, 2023
概括
在糖醇和甲醇上研究了Pichia pastoris生长动力学. 转基因菌株的生长率高于野生菌株,这表明了工业应用的优化养策略.
科学领域:
- 生物技术是生物技术.
- 微生物生理学 微生物生理学
- 工业微生物学 工业微生物学
背景情况:
- 皮奇亚牧师是生产工业产品的关键酵母平台.
- 了解基因改造对P. pastoris生长的影响对于过程优化至关重要.
- 菌株特征,促进剂和蛋白质类型影响过程复杂性.
研究的目的:
- 研究和建模Pichia pastoris野生型 (WT) 和基因改造 (GM) 菌株在甘油和甲醇上的生长动力学.
- 确定描述基质消耗和生长抑制的最佳运动模型.
- 为了比较WT和GM P. pastoris菌株的生长率.
主要方法:
- 批量种植P. pastoris GS115 WT和转基因菌株.
- 对糖醇和甲醇的增长率的估计.
- 使用Monod,Haldane和枢纽参数模型进行动力建模.
- 对基质消费模式的分析.
主要成果:
- 在糖醇上,P. pastoris的生长遵循了哈尔丹动力学,这解释了基质抑制.
- 核心参数模型最好地描述了甲醇的增长,捕捉了甲醇的毒性.
- 与WT菌株相比,转基因菌株在甘油和甲醇上表现出显著更高的生长率.
结论:
- 哈尔丹和枢机参数模型在描述P. pastoris在糖醇和甲醇上的生长方面优于莫诺德动力学.
- 经过基因工程改造的P. pastoris菌株表现出增强的生长性能.
- 研究结果表明,在转基因菌株的食批次培养中,有可能优化碳源养策略.
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