相关实验视频

Updated: Jul 16, 2025

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

大脑衍生的神经营养因子通过通过蛋白激酶A刺激线粒体功能来保护神经元

Maryann Swain¹, Smijin K Soman¹, Kylea Tapia¹

¹Department of Pharmacology, University of Nevada, Reno School of Medicine, Reno, Nevada, USA.

Journal of neurochemistry

|September 9, 2023

概括

大脑衍生神经营养因子 (BDNF) 通过其受体TrkB和蛋白质激酶A (PKA) 信号传递来改善线粒体功能,从而增强神经保护,从而提高神经元健康.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
线粒体生理学线粒体生理学

背景情况:

大脑衍生神经营养因子 (BDNF) 对神经元可塑性和生存至关重要.
BDNF在调节线粒体呼吸中的作用表明它对线粒体生理有直接影响.
将BDNF与神经元中的线粒体功能联系在一起的精确分子机制需要阐明.

研究的目的:

研究BDNF刺激神经元中的线粒体功能的分子机制.
为了确定BDNF是否与TrkB受体结合并转移到线粒体.
检查蛋白激酶A (PKA) 在BDNF介导的线粒体调节中的作用.

主要方法:

同焦点显微镜和亚细胞分离以确认TrkB受体在线粒体中的定位.
时间间隔显微镜用于评估初级皮层神经元中的线粒体动态 (融合,贩运).
XF24e代谢分析仪用于测量线粒体呼吸和糖解.
药理抑制和基因操纵PKA以评估其作用.

主要成果:

治疗BDNF促进TrkB受体转移到线粒体,激活PKA.
BDNF增强了线粒体融合,前级贩运和树突性线粒体含量.
观察到线粒体呼吸和糖溶解的增加,需要PKA活动.
在线粒体动力学上BDNF介导的影响涉及PKA依赖的Drp1和Miro-2的酸化.
治疗BDNF增加了神经元对氧化应激的抵抗力和罗诺诱导的树收缩.

结论:

通过TrkB受体转位和PKA信号传递,BDNF直接调节神经元线粒体结构和功能.
这种BDNF诱导的线粒体健康增强有助于对抗氧化应激的神经保护.
这项研究揭示了BDNF介导的神经保护的新机制,包括改善线粒体动力学和功能.

关键词:

这就是为什么BDNF是BDNF.这就是PKA PKA.这就是为什么TrkBB.生物能源生物能源学线粒体的动力学线粒体人口贩运活动

更多相关视频

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Published on: September 15, 2014

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Published on: July 11, 2020

相关概念视频

Enzyme-linked Receptors

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...

The Blood-brain Barrier

The Blood-brain Barrier

Neurogenesis and Regeneration of Nervous Tissue

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...