相关实验视频

Updated: Jul 16, 2025

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Published on: October 29, 2013

通过移植制备的多 () -蛋白结合物用于ROMP作为PEG的替代品

Elizabathe Davis¹, Adam A Caparco¹, Nicole F Steinmetz^1,2,3,4,5,6

¹Department of NanoEngineering, University of California, San Diego, La Jolla, CA, 92093, USA.

Macromolecular bioscience

|September 9, 2023

概括

新的聚氧化 (PONB) 聚合物为生物结合提供了聚乙烯糖醇 (PEG) 的替代品. 这些新型聚合物降低了对生物制剂的免疫反应,解决了对现有的PEGylated治疗方法的担忧.

科学领域:

生物结合化学生物结合化学
聚合物科学聚合物科学
免疫学免疫学免疫学

背景情况:

聚乙烯糖醇 (PEG) 化是改善生物循环时间和降低免疫性的标准.
人类中抗PEG抗体的流行率不断增加,这对PEG化治疗药物的安全性构成了挑战.
需要使用具有改善免疫特征的替代聚合物.

研究的目的:

合成新的氨基胺反应性聚氧化 (PONB) 阻断共聚合物.
评估基于PONB的合物 (PONB-PEG和PONB-Zwit) 的蛋白质生物活性和免疫性.
将PONB结合物与传统的PEGylated蛋白质进行比较.

主要方法:

环开元化聚合 (ROMP) 用于合成PONB块共聚合物.
移植到生物结合技术,将聚合物附着在lyszyme (Lyz) 和尿酸氧化酶 (UO) 上.
测试以评估结合生物活性和免疫识别.

主要成果:

合成的PONB-PEG和PONB-Zwit块共聚合物.
利兹-PONB和UO-PONB结合物保留了显著的生物活性.
与PEGylated UO相比,UO-PONB结合物显示免疫性降低.
PONB-Zwit结合物表现出可以忽略不计的PEG特异性免疫识别.

结论:

基于PONB的聚合物是一种可行的替代PEG生物结合的替代品.
这些聚合物保持生物活性,同时改善免疫识别配置文件.
在治疗性蛋白质中,PONB-Zwit为降低免疫性提供了一个有前途的选择.

关键词:

在 ROMP ROMP 里面.这是一种抗PEG的药物.生物结合物是一种生物结合物.聚 ((oxanorbornene)) 氧化基的使用情况.

更多相关视频

Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions

Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions

Published on: October 10, 2013

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

相关概念视频

Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)

Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)

Ring-opening metathesis polymerization or ROMP involves strained cycloalkenes as starting materials. The mechanism of ROMP proceeds by reacting cycloalkene with Grubbs catalyst to give metallacyclobutane intermediate which undergoes a ring-opening reaction to form new carbene. The new carbene reacts with another molecule of cycloalkene. Repetition of these steps leads to the formation of an unsaturated open-chain polymer product. All these steps are reversible, however, relieving the ring...

Olefin Metathesis Polymerization: Overview

Olefin Metathesis Polymerization: Overview

Recently, the development of olefin metathesis polymerization advanced the field of polymer synthesis. Simply put, the reorganization of substituents on their double bonds between two olefins in the presence of a catalyst is known as the olefin metathesis reaction. The use of metathesis reaction for polymer synthesis is called olefin metathesis polymerization.
Ruthenium-based Grubbs catalyst is the most commonly used catalyst for olefin metathesis polymerization. Grubbs catalyst consists...

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...