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相关概念视频

Olfactory Receptors: Location and Structure01:03

Olfactory Receptors: Location and Structure

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The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...
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相关实验视频

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Whole Mount Labeling of Cilia in the Main Olfactory System of Mice
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Whole Mount Labeling of Cilia in the Main Olfactory System of Mice

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从小鼠嗅觉球泡中捕获的目标捕获mRNA.

Kevin W Zhu1, Hiroaki Matsunami1,2

  • 1Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, USA.

Methods in molecular biology (Clifton, N.J.)
|September 9, 2023
PubMed
概括

研究人员使用一种新的空间转录学方法绘制了小鼠嗅觉球泡 (OB) 中的嗅觉受体 (OR). 这种技术成功地绘制了86%的OR,揭示了受体序列和质状体位置之间的联系.

科学领域:

  • 神经科学是一个神经科学.
  • 基因组学就是基因组学.
  • 感官系统生物学 生物学

背景情况:

  • 嗅觉依赖于有组织的感觉受体系统,嗅觉处理的中心是嗅球 (OB) 质体.
  • 了解嗅觉受体 (OR) 与淋巴细胞的映射是解读嗅觉系统结构和功能的关键.

研究的目的:

  • 开发和应用一种方法来丰富小鼠OB的低丰富的OR信使RNA (mRNA).
  • 为了生成高通量空间映射ORs到OB内的质细胞.

主要方法:

  • 组合的顺序切割 (前后,背中,中侧轴) 与目标捕获丰富序列.
  • 克服了低丰度目标基因表达的挑战,以准确地绘制.

主要成果:

  • 空间映射了整个嗅球的86%的嗅觉受体.
  • 发现了嗅觉受体序列与其在OB中的特定球粒状位置之间的相关性.

结论:

  • 开发的方法可以在OB中对OR进行全面的空间映射.
  • 揭示了嗅觉系统中与空间组织相关的OR序列的潜在组织原理.
关键词:
基因表达分析 基因表达分析嗅觉灯泡是一种嗅觉灯泡.嗅觉受体是一种嗅觉受体.有关RNA测序的RNA测序

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Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals
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相关实验视频

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